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| Description | This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. It is provided in a non-viral vector (plasmid) for quick and easy transfection. Transfection will result in transient expression of Cas9, leading to reduced chances of off-target editing. The Cas9 provided in this non-viral vector is spCas9 from Streptococcus pyogenes. |
|---|---|
| SKU | K095 |
| System | Non-Viral Vector |
| Product Applications |
|
| Vector Map | pNV-EF1a-Cas9-2A-GFP |
| Unit quantity | 10 μg |
| Promoter | EF1a |
| Cas Type | Nuclease |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Note | abm"s sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant). |
| Shipping Conditions | Shipped at ambient temperature. |
| Bacterial Selection | Ampicillin |
| Format | Plasmid |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when at -20°C or below in a non-frost free freezer) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
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