产品资料

RayBiotech/Human EGFR Phosphorylation Array C1/2 Sample Kit/AAH-PER-1-2

Antigen Information

Gene Symbols:

EGFR
ERBB2
ERBB3
ERBB4

Assay Format

Pathway:

HER/ErbB Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
EGFR(P-Tyr845) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR(P-Tyr992) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y992) ELISA	EGFR(P-Tyr1045) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y1045) ELISA	EGFR(P-Tyr1068) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y1068) ELISA	EGFR(P-Tyr1086) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (Y1086) ELISA
EGFR(P-Tyr1148) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR(P-Tyr1173) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR(P-Ser1046/1047) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533	EGFR(P-Ser1070) Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human/Mouse/Rat Phospho-EGFR (S1070) ELISA	ErbB2(P-Tyr877) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626
ErbB2(P-Tyr1112) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2(P-Tyr 1221/1222) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2(P-Tyr1248) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2(P-Thr686) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626	ErbB2(P-Ser1113) Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626
ErbB3(P-Tyr1289) Gene Symbol: ErbB3 Gene ID: 2065 Accession #: P21860	ErbB4(P-Tyr1284) Gene Symbol: ErbB4 Gene ID: 2066 Accession #: Q15303

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human EGFR Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail Set I
Phosphatase Inhibitor Cocktail II
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE"s ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Wang X., Zhang M., Ping F., et al. Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma. Hepatology. 2018 Jan 21. doi: 10.1002/hep.29792. Species: HumanSample type: Cell Lysate
Ruiz-Saenz A., Dreyer C., Campbell M., et al. HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3. Cancer Research, epub online 2018, doi: 10.1158/0008-5472.CAN-18-0430Species: HumanSample type: Cell Lysate
Tang Q., Wang Y., Ma L., et al. Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway. Oncol Rep. 2018 Mar;39(3):1299-1305. doi: 10.3892/or.2018.6184.Species: HumanSample type: Cell Lysate
Asif M., Shafaei A., Abdul Majid A., et al. Mesua ferrea stem bark extract induces apoptosis and inhibits metastasis in human colorectal carcinoma HCT 116 cells, through modulation of multiple cell signalling pathways. Chin J Nat Med. 2017 Jul;15(7):505-514. doi: 10.1016/S1875-5364(17)30076-6.Species: HumanSample type: Cell Lysate
Kato K., Iwama H., Yamashita T., et al. The anti-diabetic drug metformin inhibits pancreatic cancer cell proliferation in vitro and in vivo: Study of the microRNAs associated with the antitumor effect of metformin. Oncol Rep. 2016 Mar;35(3):1582-92. doi: 10.3892/or.2015.4496Species: HumanSample type: Tissue Lysate
Mohan N., Shen Y., Endo Y., et al. Trastuzumab, but Not Pertuzumab, Dysregulates HER2 Signaling to Mediate Inhibition of Autophagy and Increase in Reactive Oxygen Species Production in Human Cardiomyocytes. Mol Cancer Ther. 2016 Jun;15(6):1321-31. doi: 10.1158/1535-7163.MCT-15-0741.Species: HumanSample type: Cell Lysate
Rios-Doria J., Sabol D., Chesebrough J., et al. A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways. Mol Cancer Ther. 2015 Jul;14(7):1637-49. doi: 10.1158/1535-7163.MCT-14-1040. Species: HumanSample type: Tissue Lysate
Montermini L., Meehan B., Farnier D., et al. Inhibition of oncogenic epidermal growth factor receptor kinase triggers release of exosome-like extracellular vesicles and impacts their phosphoprotein and DNA content. J Biol Chem. 2015 Oct 2;290(40):24534-46. doi: 10.1074/jbc.M115.679217. Species: HumanSample type: Extracellular Vesicles
Arnold C., et al. Rac1 as a multifunctional therapeutic target to prevent and combat cancer metastasis. Oncoscience 2014, Vol.1, No.8Species: HumanSample type: Cell Lysate
Panupinthu N., et al. Self-reinforcing loop of amphiregulin and Y-box binding protein-1 contributes to poor outcomes in ovarian cancer. Oncogene (2014) 33, 2846-2856; doi:10.1038/onc.2013.259Species: HumanSample type: Cell Lysate

123

I used this array to test the cell lysates from epidermoid carcinoma cell line exposed to EGF, it works well.

Guang Wang04/24/2018, 08:38 PM

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