Gene Symbols:- CCL2
- CXCL8
- IFNG
- IL10
- IL13
- IL1A
- IL1B
- IL4
- IL6
- TNF
Pathway:- JAK/STAT Signaling
- MAPK Signaling
- NFkB Signaling
Number of Targets Detected:10
Compatible Sample Types:- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Method of Detection:Fluorescence Laser Scanner
Quantitative/Semi-Quantitative: Solid Support:Glass Slide
Size:1, 2, or 4 Slide Kit (16 sub-arrays per slide)
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
| Scroll over each target protein for more information |
|---|
IFN-gamma- Gene Symbol: IFNG
- Gene ID: 3458
- Accession #: P01579
- ELISA Kit: RayBio Human IFN-gamma ELISA
| IL-1 alpha(IL-1 F1)- Gene Symbol: IL1A
- Gene ID: 3552
- Accession #: P01583
- ELISA Kit: RayBio Human IL-1 alpha ELISA
| IL-1 beta(IL-1 F2)- Gene Symbol: IL1B
- Gene ID: 3553
- Accession #: P01584
- ELISA Kit: RayBio Human IL-1 beta ELISA
| IL-10- Gene Symbol: IL10
- Gene ID: 3586
- Accession #: P22301
- ELISA Kit: RayBio Human IL-10 ELISA
| IL-13- Gene Symbol: IL13
- Gene ID: 3596
- Accession #: P35225
- ELISA Kit: RayBio Human IL-13 ELISA
|
IL-4- Gene Symbol: IL4
- Gene ID: 3565
- Accession #: P05112
- ELISA Kit: RayBio Human IL-4 ELISA
| IL-6- Gene Symbol: IL6
- Gene ID: 3569
- Accession #: P05231
- ELISA Kit: RayBio Human IL-6 ELISA
| IL-8(CXCL8)- Gene Symbol: IL8
- Gene ID: 3576
- Accession #: P10145
- ELISA Kit: RayBio Human IL-8 ELISA
| MCP-1(CCL2)- Gene Symbol: CCL2
- Gene ID: 6347
- Accession #: P13500
- ELISA Kit: RayBio Human MCP-1 ELISA
| TNF alpha- Gene Symbol: TNF
- Gene ID: 7124
- Accession #: P01375
- ELISA Kit: RayBio Human TNF-alpha ELISA
|
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
Learn MoreProtocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Manual
SDS
GAL
Analysis Tool