产品资料

RayBiotech/Human Angiogenesis Array Q1000/8 Sample Kit/QAH-ANG-1000-1

Antigen Information

Gene Symbols:

AGRP | ANG | ANGPT1 | ANGPT2 | ANGPTL4 | CCL1 | CCL13 | CCL2 | CCL5 | CCL7 View more Hide

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 100 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Activin A Gene Symbol: INHBA Gene ID: 3624 Accession #: P08476 ELISA Kit: RayBio Human Activin A ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA	Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA
Angiostatin Gene Symbol: PLG Gene ID: 5340 Accession #: P00747 ELISA Kit: RayBio Human Angiostatin ELISA	ANGPTL4 Gene Symbol: ANGPTL4 Gene ID: 51129 Accession #: Q9BY76 ELISA Kit: RayBio Human ANGPTL4 ELISA	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA
ENA-78(CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	FGF-4 Gene Symbol: FGF4 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA	Follistatin Gene Symbol: FST Gene ID: 10468 Accession #: P19883 ELISA Kit: RayBio Human Follistatin ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA
GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876	HB-EGF Gene Symbol: HBEGF Gene ID: 1839 Accession #: Q99075 ELISA Kit: RayBio Human HB-EGF ELISA	HGF Gene Symbol: HGF Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	I-309(TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552 ELISA Kit: RayBio Human IL-17A ELISA
IL-1 alpha(IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta(IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA
IL-8(CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IP-10(CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	I-TAC(CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA	Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA	LIF Gene Symbol: LIF Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA
MCP-1(CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2(CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3(MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	MCP-4(CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	MMP-1 Gene Symbol: MMP1 Gene ID: 4312 Accession #: P03956 ELISA Kit: RayBio Human MMP-1 ELISA
MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780 ELISA Kit: RayBio Human MMP-9 ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	PECAM-1(CD31) Gene Symbol: PECAM1 Gene ID: 5175 Accession #: P16284 ELISA Kit: RayBio Human PECAM-1 ELISA	PLGF Gene Symbol: PGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA	RANTES(CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA
TGF alpha Gene Symbol: TGFA Gene ID: 7039 Accession #: P01135 ELISA Kit: RayBio Human TGF-alpha ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	Tie-1 Gene Symbol: TIE1 Gene ID: 7075 Accession #: P35590 ELISA Kit: RayBio Human Tie-1 ELISA	Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763 ELISA Kit: RayBio Human Tie-2 ELISA
TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta(TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	Thrombopoietin(TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA
uPAR Gene Symbol: PLAUR Gene ID: 5329 Accession #: Q03405 ELISA Kit: RayBio Human uPAR ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916 ELISA Kit: RayBio Human VEGF R3 ELISA	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915 ELISA Kit: RayBio Human VEGF-D ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Angiogenesis Array Q1000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Schuster C., Akslen L., Stokowy T., Straume O. Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy. J Pathol Clin Res. 2019 Jan;5(1):53-62. doi: 10.1002/cjp2.116. Species: HumanSample type: Serum
Zbucka-Kretowska, Monika, et al. "Amniotic fluid angiogenic and inflammatory factor profiling in foetal down syndrome."?Fetal diagnosis and therapy?44 (2018): 44-50.Species: HumanSample type: Amniotic fluid
Chen J, Liu Z, Hong MM, Zhang H, Chen C, et al. (2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells. PLoS ONE 9(12): e115316. doi:10.1371/journal.pone.0115316Species: HumanSample type: Cell Lysate
Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regenerationSpecies: HumanSample type: Conditioned Media
Storti, P., et al. "HOXB7 expression by myeloma cells regulates their pro-angiogenic properties in multiple myeloma patients."?Leukemia?25.3 (2011): 527-537.Species: HumanSample type: Cell Lysate
Gujar, Shashi A., et al. "Oncolytic virus-initiated protective immunity against prostate cancer."?Molecular Therapy?19.4 (2011): 797-804.Species: HumanSample type: Conditioned Media

It was hard to use at first but once you get the gist of how to use it, it becomes easier, I've been using it since 2017 to detect proteases on our artificial environment and works great! Recommended.

Ruston PerryNASA - Biology11/16/2019, 10:03 AM

This product is simple to use with step by step instructions. It also has multiple options for an overnight incubation, which can be beneficial on a busy day!

JoehleenUC Davis12/19/2018, 03:57 PM

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