产品资料

RayBiotech/Human 15-PGDH/HPGD ELISA/1 Plate Kit/ELH-15PGDH-1

Antigen Information

Accession Number:

P15428

Gene ID:

3248

Gene Symbols:

HPGD
PGDH1
SDR36C1

Protein Name & Synonyms:

15-hydroxyprostaglandin dehydrogenase [NAD(+)] (15-PGDH) (EC 1.1.1.141) (Prostaglandin dehydrogenase 1) (Short chain dehydrogenase/reductase family 36C member 1)

Target Species:

Human

Assay Format

Detection Range:

0.2 ng/ml - 50 ng/ml

Recommended Dilution (serum & plasma):

2 fold

Sensitivity:

0.2 ng/ml

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Specificity:

This ELISA antibody pair recognizes Human 15-PGDH.

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants

Design Principle:

Sandwich-based

Method of Detection:

Colorimetric

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

96-well Microplate

Product Specifications

Size:

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

The only thing I would improve is the incubation time.

CorrineUT01/04/2019, 03:08 PM

Assay worked well for human cancer cells (MDA-MB)

William04/07/2018, 10:57 PM

The ELISA is sensitive enough to detect the 15 PGDH inth cells. All the enssential kits and solutions are also included in the package. However, it would be bettter if the incubation time can be reduced.

andy lee04/05/2018, 01:39 PM

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