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| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 재고 | 재고 | 수량 |
|---|
T4-β-glucosyltransferase는 5-hydroxymethylcytosine 잔기의 5 - hydroxyl methly기에 UDP-Glucose (uridine diphosphoglucose)의 glucose일부를 전이하는 효소이다.
50 × UDP-Glucose25㎕10 × T4-BGT Buffer 120㎕
20 mM | Potassium Phosphate (pH7.0 at 25 ℃) |
0.2 M | NaCl |
0.1 mM | EDTA |
0.25 mM | DTT |
50 % | Glycerol |
T4-β-glucosyltransferase | 1 μl |
10 × T4-BGT Buffer | 3 μl |
50 × UDP-Glucose | 0.6 μl |
Substrate DNA |
|
멸균 증류수 | up to 30 μl |
↓ | |
37 ℃에서 1 시간 배양 | |
1. 포유류와 식물 게놈 DNA에 존재하는 5-hydroxymethylcytosine의 glycosylation 2. Glycosylation시킨 5-hydroxymethylcytosine sensitive enzyme과의 조합에 의한 5-hydroxymethylcytosine 검출
50 × UDP-Glucose2 mM UDP-Glucose10 × T4-BGT Buffer100 mM Tris-HCl (pH7.9 at 25 ℃)500 mM NaCl100 mM MgCl210 mM DTT
-20 ℃
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1 μg