Chimerx/CviJ I/2125/100 u

Source

Chlorella virus IL-3A
Note 1: Purified from E coli strain that carries the cloned cvijRI gene from Chlorella virus IL-3A (Patent No. US005472872A)

Reagents Supplied

10X CviJ I Reaction Buffer
DMSO 

Applications

• CviJ I is the only available restriction endonuclease recognizing two-three base pair sequence (1,2,3), thus allowing for new research applications (4)
• This recombinant version of CviJ I cleaves only PuGCPy sites (4)
• Suitable for high resolution mapping of short DNAs like amplified products or small plasmids
• Other CviJ I applications, like shotgun cloning, thermal cycle labeling (5) or epitope mapping can be performed either with this version or with CviJ I*
• Exclusively from CHIMERx

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of pBR322 DNA in 1 hour in a total reaction volume of 25 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

pBR322

Reaction Buffer

10X CviJ I Reaction Buffer:
20 mM glycylglycine-KOH (pH 8.5)
10 mM magnesium acetate
50 mM potassium acetate
0.1 mM dithiothreitol
Note 2: 100% DMSO supplied separately

Assay Conditions

20 mM glycyl-glycine-KOH (pH 8.5)
50 mM potassium acetate
10 mM magnesium acetate
0.1 mM dithiothreitol
10% DMSO
1 µg pBR322

Incubation for 1 hour at 37°C in a reaction volume of 25 µl

Storage Buffer

20 mM Tris-acetate (pH 8.0 at 4°C)
50 mM potassium acetate
5 mM MgCl₂
0.5 mM EDTA
1 mM dithiothreitol
50% (v/v) glycerol

Storage Conditions

Store at -80°C
Shipped on dry ice

Downloads

Cerftificate of Analysis PDF - Current Lot

References

(1) Xia,Y., Burbank, D., Uher, L., Rabussay, D. and Van Etten, J. Nucleic Acids Res.15, 6075-6090
(2) Fitzgerald,M.C., Skowron, P., Van Etten, J.L., Smith, L.M. and Mead, D.A. (1992) Nucleic Acids Res. 20, 3753-3762
(3) Mead, D., Swaminathan, N., Van Etten J. and Skowron, P.M.: Recombinant CviJI restriction endonuclease. (1995) Unites States Patent no US005472872A
(4) Skowron, P.M, Swaminathan, N., McMaster, K., George, D., Van Etten, J. and Mead, D. Gene 157 (1995) 37-41
(5) Swaminathan, N., McMaster, K., Skowron, P. and Mead, D.A. Analytical Biochemistry 255 (1998) 133-141