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Chimerx/RNase Inhibitor/4210/10,000 u

Details
Description
  • MorepotentthancompetinghumanplacentalRNaseinhibitors
  • RNase-free
  • EffectiveoverbroadpHrangefrompH5.5to8.5
  • Activeovertemperaturerangefrom37°Cto70°C
  • RNaseInhibitordoesnotinterferewithSP6,T7,orT3RNApolymerase,AMVorM-MLVreversetranscriptaseorTaqDNApolymerase
  • RNaseInhibitorincreasesthetimeRNAcanbesafelystored
UnitDefinition

Oneunitistheamountofribonucleaseinhibitorrequiredtoinhibittheactivityof5ngofRibonucleaseAat25°C(by50%)
RibonucleaseinhibitoractivityisdeterminedbytheinhibitionofRNaseAhydrolysisofcyclic2",3"-cytdinemonophosphateinaspectrophotometerrecordingassay

StorageBuffer

20mMHepes-KOH(pH7.5)
100mMKCl
0.1mMEDTA
50%(v/v)glycerol

AssayConditions

0.1MTris-acetate(pH6.5at22°C)
1mMEDTA
1mMcyclic2",3"-cytidinemonophosphate
Reactionvolumeof1ml

QualityControl

AllpreparationsaretestedforcontaminatingendonucleaseandexonucleaseandnonspecificRNaseandsingle-anddouble-strandedDNaseactivites

StorageContitions

Storeat-20°C
ProductshippedondryIce

Downloads

CertificateofAnalysisPDF-CurrentLot
MSDSPDF

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