Chimerx/Pvu II/2330/5,000 u

Pvu II Source

Proteus vulgaris

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

No

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂ 
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 75%
Buffer 4: 75%
Buffer 5: 75%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.4 at 4°C)
50 mM KCl
0.1 mM EDTA
10 mM BME
500 µg/ml bovine serum albumin
0.1% Tween 20™
50% (v/v) glycerol

Assay Conditions 

10 mM Tris-HCl (pH 7.5 at 37°C)
50 mM NaCl
10 mM MgCl₂ 
1 mM dithiothrietol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Note

To avoid star activity it is not recommended to use more than 15 units per reaction or to incubate over 1 hour

Storage Conditions

Store at -20°C
Shipped on dry ice 

Pvu II Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases