Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
Chimerx/TspDT I/2502/250 u188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

Chimerx/TspDT I/2502/250 u

Details
TspDTISource

PurifiedfromEcolistrainthatcarriestheclonedtspDTRIgenefromThermusspeciesDT

UnitDefinition

Oneunitistheamountofenzymerequiredtocompletelydigest1 µgofpUC19DNAtoobtainastabledigestionpatternin1hourinatotalreactionvolumeof30 µl

StarActivity

N/A

ReactionTemperature

70°C

HeatInactivation

No

AssayUnitSubstrate

pUC19DNA

ReactionBuffer

10mMTris-HCl(pH8.5at25°C)
10mMMgCl2
1mMdithiothreitol
+Enhancers(1)

Notes:

  1. Avoidmultiplecyclesoffreezing/thawingofthestockreactionbuffer;Nomorethanthree[3]times.
  2. Thawingshouldbepreformedattemperaturesnotexceeding10°C.
  3. Recommendedprocedureistodividetheprovidedreactionbufferintosmallerportionsandpreservethemat-70°Cforlongtermstorage.
  4. Recommendedshorttermstorageofreactionbufferisat    -20°C.
StorageBuffer

20mMTris-HCl(pH8.3at25°C)
25mM(NH4)2SO4 
25mMKCl
0.5mMEDTA
0.5mMdithiothreitol
0.02%TritonX-100™
0.02%Tween20™
0.02%Igepal
50%(v/v)glycerol

AssayConditions 

10mMTris-HCl(pH8.5at25°C)
10mMMgCl2 
1mMdithiothreitol 
1 µgpUC19DNA
+Enhancers(1)
Incubationisat70°Cfor1hourinareactionvolumeof30 µl 

Notes
  1. Itisnotrecommendedtousemorethan2unitsper30 µlreaction.Itissuggestedtoperformdigestionforover1hour.
  2. ToavoidDNAshiftduringelectrophoresiscausedbystrongprotein-DNAinteraction,itisrecommendedtoterminatereactionbyadditionofreactionstopsolution(containingdenaturingreagent,i.e.0.2%SDS)followedbyheatinactivationat89°Cfor20minutes.
StorageConditions

Storeat-20°C
Shippedondryice

References

(1)Skowron,P.M.,Majewski,J.,Zylicz-Stachula,A.,Rutokowska,S.M.,Jaworowska,I.,andHarasimowicz-Slowinska,R.I.(2003)NucleicAcidsResearch31,14e74

Downloads

CertificateofAnalysisPDF-CurrentLot
MSDSPDF-CurrentLot

Categories

RestrictionEndonucleases

      相关产品

      matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY

      intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15

      goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP

      novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg

      novateinbio/SARS-Associated Coronavirus Spike Mosaic S ( C ) Recombinant ( SARS Mosaic S ( C ) )/500ug/PT_42025_500ug

      matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB

      intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24

      goprolytix//10 ug, 100 ug/RTF-0300

      最新签约

      Liposoma

      KAMIYA

      Bioclone

      intactgenomics

      Abbexa

      Emfret

      Tribioscience

      goprolytix

      技术文章

      ProChimia/HS(CH2)mCOOH/FT 002m101/1 g

      Alomone经销商/代理商 Alomone蛋白质

      prospec mSCF

      【供应 GelRed核酸染料 （Biotium 41003）】价格_厂家 苏州蚂蚁淘

      PDMS Device Fabrication and Surface Modification | Protocol

      prospec HOR 250

      上海正品NSJ Bioreagents生物试剂1 NSJ Bioreagents细胞生物学

      杭州Enzymatics inc批发，产品货源 Enzymatics T4连接酶

      新闻动态

      苏州Enzymatics型号参数规格 Enzymatics超纯poly酶

      19413561 GAMMASECRETASE INHIBITOR X 1MG

      合肥中国Abbexa官网，Abbexa Ltd价格更新 abbexa授权代理

      Abbexa中国采购 abbexa纯化蛋白

      RhodamineDHPE美国/Biotium询价/索取资料——苏州蚂蚁淘

      武汉美国Polysciences公司 分析试剂Polysciences

      广州Anatrace型号参数规格 Anatrace膜蛋白提取与纯化

      Genovis 特约代理苏州蚂蚁淘实验试剂有限公司

      行业前沿

      正品Pfenex生物试剂 重组白喉杆菌CRM197,为什么这么便宜?

      PfenexX中国代理价格，厂家 SEA133鲑鱼纤维蛋白原,求推荐!

      Pfenex中国采购 三文鱼凝血酶/鲑鱼凝血酶 Salmon Thrombin,是正品吗?

      Polysciences聚乙烯亚胺 Polyethylenimine,好用吗?

      PolysciencesX中国代理价格，厂家 Polysciences24765,哪里有卖?

      Polysciences代理，产品目录 Polysciences聚合物,是正品吗?

      Sea Run Holding产品促销 重组白喉杆菌CRM197,为什么这么便宜?

      生命科学Pfenex价格优惠 Sea Run Holding代理，产品目录,有优惠活动吗?

      最热产品

      Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003

      Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003

      Qubit™ dsDNA BR Assay Kit Q32853现货

      Parafilm M封口膜/PM996/4x125/整箱

      Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销

      LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货

      GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g

      Leica徕卡819一次性窄刀片【50片/盒，10盒/条】/14035838925

      推荐新闻

      RA Biosources报价单 rabios代理

      Lampire型号参数规格 Lampire公司

      品牌试剂实验Medica Lisotopes代理 Medica Lisotopes产品

      进口试剂QoolAbs直采 Qoolabs噬菌体筛选

      进口Molecular Research Center品牌清关代理 MRC一步法TRIzol RNA抽提

      授权代理商，正品 Spherefluidics授权

      Ursabioscience美元价/进货价 Ursa BioScience产品

      Wards Science抗体，试剂，血清，细胞 wardsci授权

      新闻动态
      行业前沿
      技术文章
      最新产品