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产品资料

Chimerx/Stu I/2405/1,000 u

Details
StuISource

Streptomycestubercidicus

ReagentsSupplied

CMXBuffer2

UnitDefinition

Oneunitistheamountofenzymerequiredtocompletelydigest1µgofumethylatedlamBDaDNAin1hourat37°Cinatotalreactionvolumeof50µl

StarActivity

No

ReactionTemperature

37°C

HeatInactivation

10minutesat65°C 

AssayUnitSubstrate

LambdaDNA

ReactionBuffer

CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin

ActivityinBuffers

Buffer1: 75%
Buffer2: 100%
Buffer3:50%
Buffer4:75%
Buffer5:75%

StorageBuffer

10mMTris‑HCl(pH7.4at4°C) 
50mMKCl
0.1mMEDTA
1mMdithiothreitol
0.5mg/mlbovineserumalbumin
0.1mMPMSF
50%(v/v)glycerol

AssayConditions

10mMTris-HCl(pH7.5at37°C)
50mMNaCl
10mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µgunmethylatedlambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl

StorageConditions

Storeat-20°C
Shippedondryice 

Downloads

CertificateofAnalysis PDF-CurrentLot
MSDSPDF -CurrentLot

Categories

RestrictionEndonucleases

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