产品资料

ACROBiosystems/Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™/200ug/AC2

Synonym
ACE-2,ACEH,ACE2
Source
Biotinylated Human ACE2, Fc,Avitag (AC2-H82F9) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
Predicted N-terminus: Gln 18
Request for sequence
Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 111.7 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. The product MUST be stored at -70°C or lower upon receipt;
2. -70^°C for 3 months under sterile conditions.
Shipping
This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.

SDS-PAGE

Biotinylated Human ACE2, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-BLI

Loaded Biotinylated Human ACE2, Fc,Avitag (Cat. No. AC2-H82F9) on SA Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 31.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Background
Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.
References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ACROBiosystems/Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™/200ug/AC2