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ACROBiosystems/Human Chitinase 3-like 1 / CHI3L1 Protein, His Tag/1mg (500ug × 2)/CH1

  • Synonym
    CHI3L1,YKL-40,CGP-39,GP-39,ASRT7
  • Source
    Human CHI3L1, His Tag (CH1-H5228) is expressed from human 293 cells (HEK293). It contains AA Tyr 22 - Thr 383 (Accession # AAH38354).
    Predicted N-terminus: Tyr 22
    Request for sequence
  • Molecular Characterization
    Online(Tyr 22 - Thr 383) AAH38354

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.2 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CHI3L1, His Tag (Cat. No. CH1-H5228) SDS-PAGE gel

Human CHI3L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Chitinase-3-like protein 1 (CHI3L1) is also known as Cartilage glycoprotein 39 (CGP-39), YKL-40, which belongs to the glycosyl hydrolase 18 family. CHI3L1 is present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. CHI3L1 may play a role in defense against pathogens, or in tissue remodeling and also may play an important role in the capacity of cells to respond to and cope with changes in their environment.
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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