产品资料

ACROBiosystems/Human CEACAM-5 / CD66e Protein, His Tag (MALS verified)/1mg (500ug × 2)/CE5

Synonym
CEACAM-5,CD66e,CEA,Meconium antigen 100
Source
Human CEACAM-5, His Tag (CE5-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731).
Predicted N-terminus: Lys 35
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 72.2 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human CEACAM-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human CEACAM-5, His Tag (Cat. No. ) MALS images

The purity of Human CEACAM-5, His Tag (Cat. No. CE5-H5226) was more than 95% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

Report

Bioactivity-ELISA

Human CEACAM-5, His TagHuman CEACAM-5, His Tag (Cat. No. CE5-H5226) ELISA bioactivity

Immobilized Human CEACAM-5, His Tag (Cat. No. CE5-H5226) at 1 μg/mL (100 μL/well) can bind Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Protocol

Immobilized Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 Antibody at 1 μg/mL (100 μL/well) can bind Human CEACAM-5, His Tag (Cat. No. CE5-H5226) with a linear range of 10-39 ng/mL (Routinely tested).

Protocol

Background
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.
References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ACROBiosystems/Human CEACAM-5 / CD66e Protein, His Tag (MALS verified)/1mg (500ug × 2)/CE5