产品资料

ACROBiosystems/Human LSECtin / CLEC4G Protein, Fc Tag/50ug/CLG

Synonym
CLEC4G,LSECtin
Source
Human LSECtin, Fc Tag (CLG-H5250) is expressed from human 293 cells (HEK293). It contains AA Lys 55 - Cys 293 (Accession # Q6UXB4-1).
Predicted N-terminus: Glu
Request for sequence
Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 52.1 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>85% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human LSECtin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Citations
- Characterization of a novel anti-human lymphocyte activation gene 3 (LAG-3) antibody for cancer immunotherapy
  Authors: X Yu, et al
  Journal: MAbs 43642
  Application: ELISA
  Request for Full-text
- Antibodies binding lag-3 and uses thereof
  Authors: X Kang, et al
  Journal: US20190016800A1 43482
  Application: ELISA
  Request for Full-text
Background
C-type lectin superfamily 4, member G (CLEC4G) is also known as liver and lymph node sinusoidal endothelial cell C-type lectin (LSECtin), which is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily. CLEC4G / LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner. In addition, CLEC4G / LSECtin has the ability to bind to surface glycoproteins of enveloped viruses. In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin-mediated infection of cells by Ebola virus has been demonstrated.
References
- (1)Liu W., et al., 2004, J Biol Chem. 279(18):18748-58.
- (2)Powlesland AS., et al., 2008, J Biol Chem. 283(1):593-602.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ACROBiosystems/Human LSECtin / CLEC4G Protein, Fc Tag/50ug/CLG