产品资料

ACROBiosystems/Human B7-H5 / Gi24 / VISTA Protein, His Tag (MALS verified)/1mg/B75

Synonym
B7-H5,SISP1,Gi24,VISTA
Source
Human B7-H5, His Tag (B75-H52H0) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568).
Predicted N-terminus: Phe 33
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.0 kDa. The protein migrates as 33-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human B7-H5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human B7-H5, His Tag (Cat. No. ) MALS images

The purity of Human B7-H5, His Tag (Cat. No. B75-H52H0) was more than 95% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.

Report

Bioactivity-SPR

Human VSIG3, Fc Tag (Cat. No. VS3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human B7-H5, His Tag (Cat. No. B75-H52H0) with an affinity constant of 53.4 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Bioactivity-BLI

Loaded Human B7-H5, His Tag (Cat. No. B75-H52H0) on HIS1K Biosensor, can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 23 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

Loaded Human VSIG3, Fc Tag (Cat. No. VS3-H5258) on AHC Biosensor, can bind Human B7-H5, His Tag (Cat. No. B75-H52H0) with an affinity constant of 16 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Background
Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ACROBiosystems/Human B7-H5 / Gi24 / VISTA Protein, His Tag (MALS verified)/1mg/B75