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ACROBiosystems/Human BTN3A3 / BTF3 Protein, His Tag/1mg (250ug × 4)/BT3

  • Synonym
    BTN3A3,BTF3
  • Source
    Human BTN3A3, His Tag (BT3-H52H0) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Trp 248 (Accession # AAH15815).
    Predicted N-terminus: Gln 30
    Request for sequence
  • Molecular Characterization
    Online(Gln 30 - Trp 248) AAH15815

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.5 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BTN3A3, His Tag (Cat. No. BT3-H52H0) SDS-PAGE gel

Human BTN3A3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Butyrophilin subfamily 3 member A3 (BTN3A3),a member of the immunoglobulin superfamily and BTN/MOG family, is also known as BTF3, which contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A3 is detected in peripheral blood mononuclear cells ,T-cells ,spleen and lymphocytes. BTN3A3 also plays a role in T-cell responses in the adaptive immune response. Stimulation of human butyrophilin 3(BTN3) molecules results in negative regulation of cellular immunity.
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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