AmplideX PCR/CE FMR1 Reagents* are market-leading research tools for the detection of CGG repeats in the fragile X mental retardation (FMR1) gene. These reagents provide a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design to reliably amplify and detect all alleles including Full Mutations.
AmplideX PCR/CE FMR1 Reagents* have created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.
Reduced ComplexityEase-of-analysis of the FMR1 gene has been simplified through:
Optimized WorkflowValuable operator hands-on time has been significantly reduced through:
Quality PerformancePerforming FMR1 Analysis with Greater Sensitivity and Accuracy:
*For Research Use Only. Not for use in diagnostic procedures.
Analytical Characteristics of AmplideX PCR/CE FMR1 Reagents*:
Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal
Figure 2. Female premutation sample with accurate sizing of Normal (30 CGG) and Pre mutation allele (56 CGG)
Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity
Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX PCR/CE FMR1 Control | 24 UL | 49513 |
AmplideX mPCR FMR1 Control | 24 UL | 49514 |
AmplideX PCR/CE FMR1 Reagents | 100 | 49402 |
AmplideX mPCR FMR1 Kit | 24 | 49442 |
AmplideX PCR/CE FMR1 Reporter | N/A | 49576 |
T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com
AmplideX PCR/CE FMR1 Kit is an in vitro diagnostic (IVD) device for use in clinical laboratories for detection of the CGG repeats in the fragile X mental retardation (FMR1) gene. The device is intended to aid in the diagnosis of fragile X syndrome and fragile X associated disorders, e.g. tremor and ataxia syndrome (FX-TAS) and primary ovarian insufficiency (FXPOI), through determination of CGG repeat length up to 200 CGG and detection of alleles greater than 200 CGG. The kit provides a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design to reliably amplify and detect all alleles including Full Mutations.
AmplideX PCR/CE FMR1 Kit has created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.
Reduced ComplexityEase-of-analysis of the FMR1 gene has been simplified through:
Optimized WorkflowValuable operator hands-on time has been significantly reduced through:
Quality PerformancePerforming FMR1 Analysis with Greater Sensitivity and Accuracy:
Analytical Characteristics of AmplideX PCR/CE FMR1 Kit:
Table 1: Diagnostic Sensitivity of 100%; Diagnostic Specificity of 98.4% and Overall Accuracy of 99%*These 2 samples presented premutation alleles by both methods and low intensity full mutation alleles detected only by the AmplideX PCR/CE FMR1 Kit
Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal
Figure 2. Female Pre-mutation sample with accurate sizing of Normal (30 CGG) and Pre-mutation allele (56 CGG)
Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity
Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX PCR/CE FMR1 Control* | 24 UL | 49513 |
AmplideX mPCR FMR1 Control* | 24 UL | 49514 |
AmplideX PCR/CE FMR1 Reagents* | 100 | 49402 |
AmplideX PCR/CE FMR1 Kit | 100 | 76008 |
AmplideX mPCR FMR1 Kit* | 24 | 49442 |
AmplideX PCR/CE FMR1 Reporter* | N/A | 49576 |
T 1-877-777-1874; 512-681-5200 F 1-512-681-5202 E orders@asuragen.com
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