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Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samplesHigh Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detectionRobustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methodsCost Effective: Offered at much discounted price compared to market price
Bimake™ 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with high concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT, StepOne™, or StepOnePlus™ instruments with high sensitivity, specificity and reliability.
Two premixed versions (High ROX and Low ROX) are provided for the following qPCR instruments:
| High ROX | Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™. |
| Low ROX | Applied Biosystems 7500, 7500 Fast, ViiA™7; Stratagene MX4000™, MX3005P™, MX3000P™, QuantiStudio™ 12K Flex. |
Dye-based quantitative PCR detection Nucleic acid amplification and expression profilingQuantitative genotyping studiesGenomics-related applications
| Component | Cat #: B21402 | Cat #: B21403 |
|---|---|---|
| Bimake™ 2x SYBR Green qPCR Master Mix※(High ROX) | 5 mL (for 200 reactions with 50 μL/Rxn for 500 reactions with 20 μL/Rxn for 1000 reactions with 10 μL/Rxn) | 25 mL (for 1000 reactions with 50 μL/Rxn for 2500 reactions with 20 μL/Rxn for 5000 reactions with 10 μL/Rxn) |
| ※ Contains hot-start DNA Polymerase, dNTPs, Mg2+, SYBR Green I dye and ROX Dye (high concentration) | ||
Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.
User Guide | 2x SYBR Green qPCR Master Mix (High ROX)
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