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BreastCocktail(CKHMW/p63+CK7/8/18)iscomprisedofmousemonoclonalanti-CKHMWandanti-p63antibodiesaswellasrabbitmonoclonalanti-CK7andmousemonoclonalanti-CK8/18antibodies.CKHMW(highmolecularweightcytokeratin)isexpressedinthecytoplasmofbasalcellsandmyoepitheliumofbreasttissue(1-4).p63isatranscriptionfactorpresentinthenucleiofmyoepithelialcells(2,4).Incontrast,CK7,CK8andCK18arelowmolecularweightcytokeratinsprimarilyexpressedinluminalcellsofthebreast(1-3).
CKHMW,p63,CK7,CK8andCK18haveroutinelybeenusedasapanelofIHCMarkerstocomplementmorphologicalevaluationintheassessmentofbreastlesions,duetothedifferentialexpressionoftheluminalvs.basalandmyoepithelialmarkers(1-5).Casesofusualductalhyperplasia(UDH)havebeenassociatedwithexpressionofthebasalcellmarkers,intermixedwithcellsexpressingthekeratinsofluminalcells(1-2,6-10).Mostcasesofatypicalductalhyperplasia(ADH)andlowgradeductalcarcinomainsitu(LG-DCIS)werenegativeforthebasalmarkersandexhibitedanimmunophenotypeindicativeofluminalcells(1,5-8).Additionally,thebasalphenotypehasbeenshowntobecharacterizedbyluminalexpressionofthebasalandmyoepithelialmarkers,usingacocktailofCKHMWandp63(11-13).
IHC,usingCKHMW,p63,CK7,CK8andCK18antibodies,evaluatedincombinationwithhematoxylinandeosin(H&E),hasbeenshowntosignificantlyincreaseinter-observeragreementamongstpathologists,comparedtoH&Ealone(14).
| Format | VP |
|---|---|
| Volume | 6.0ml |
| ByLetter | B |
| IntendedUse | IVD |
| Antigen | CKHMW,CK7,CK8/18,p63 |
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