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5280:Recombinant Murine Interleukin-12 PRODUCT DESCRIPTION: Recombinant Murine IL-12 produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. The rmIL-12 is purified by proprietary chromatographic techniques. SOURCE: Baculovirus FORMULATION: The protein was lyophilized with no additives. PURITY: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE (c) Anion-exchange FPLC. STORAGE: Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. SOLUBILITY: It is recommended to reconstitute in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.BIOLOGICAL ACTIVITY: rmIL-12 is fully biologically active when compared to standard. The ED50 was determined by the stimulation of IFN-gamma production by murine splenocyes co-stimulated with IL-12 and was found to be < 0.1ng/ml, corresponding to a specific activity of >1 x 107. |
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