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5310:Recombinant Ovine Leptin (Anti-Obesity Protein) PRODUCT DESCRIPTION: Recombinant Ovine Leptin produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16,130 Dalton. The rOLeptin is purified by proprietary chromatographic techniques. SOURCE: Escherichia Coli. FORMULATION: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. PURITY: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel STORAGE: Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. SOLUBILITY: It is recommended to reconstitute the lyophilized rOLeptin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. BIOLOGICAL ACTIVITY: rOLeptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependant stimulation of Human OB-R transfected murine BaF3 indicator cells is 0.33-0.05 ng/ml. |
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610 Phone: 609-586-1575, Fax: 609-586-6892 |
FOR RESEARCH USE ONLY |
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