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4133: Recombinant Human Rantes (CCL5) Description:Recombinant Human Rantes produced in E.Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7809.2 Dalton. The rHuRantes is purified by proprietary chromatographic techniques. Source:Escherichia Coli. Formulation:Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Solubility:It is recommended to reconstitute the lyophilized rHuRantes in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Stability:Lyophilized rHuRantes although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution rHuRantes should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Purity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Tyr-Ser-Ser. Dimers and aggregates:Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity:Fully biologically active when compared to standard. The activity is determined by the chemoattract of human blood monocytes at a concentration between 1-10ng/ml. Endotoxin:Less than 0.1 ng/µg (IEU/µg) of rHuRantes. Protein content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm.2. Analysis by RP-HPLC, using a standard solution of Eotaxin as a Reference Standard. |
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