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Hooke labs/Control and naive tissue, cells, and serum/DBA/1-F/CZ-0010

Cells in vial

Control and naive tissue, cells, and serum

For analysis of gene expression and protein production

Snap frozen tissue, fresh cells, and frozen serum from naïve mice (no immunization) and control mice (immunized with adjuvant only).

These products are for use as negative controls, for comparison to disease-induced mice. Tissue is time-matched to tissue from diseased mice.

  • Analyze gene expression, protein presence, and biomarkers without a vivarium

Save time establishing & running disease models.

  • All products can be customized to your requirements

Contactorfor pricing and details.

Availability and lead time (IMPORTANT)

Most frozen products are available from stock with no lead time, but contact us atorfor current inventory.

Fresh cell orders must be placed 3 weeks before shipment. Each cell product order is custom prepared.

Note: Brain tissue is also available for some mice (add $17/mouse).See our current inventory for availability.

CIA control tissue and serum

Snap frozen tissue from mice immunized with adjuvant only (control kit; no CIA induction), matched for time from CIA induction.

Cat # Strain-Gender Termination Tissue Size Price
CZ-0010 DBA/1-F Time matched to 24-48 hrs after CIA onset Hind paw, spleen, serum Half mouse $248
CZ-0011 DBA/1-M Time matched to 24-48 hrs after CIA onset Hind paw, spleen, serum Half mouse $206
CZ-0020 DBA/1-F Time matched to 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $248
CZ-0021 DBA/1-M Time matched to 4-5 days after CIA onset Hind paw, spleen, serum Half mouse $206
CZ-0030 DBA/1-F Time matched to 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $248
CZ-0031 DBA/1-M Time matched to 2-3 weeks after CIA onset Hind paw, spleen, serum Half mouse $206

(See also CIA tissue.)

Frozen serum from mice immunized with adjuvant only (control kit).

Cat # Strain Termination Tissue Size Price
CZ-0270 DBA/1 35 to 60 days after immunization Serum 1 mL $684
CZ-5270 DBA/1 35 to 60 days after immunization Serum 250 µL $295

(See also CIA serum.)

EAE control tissue and cells

Snap frozen tissue from mice immunized with adjuvant only (control kit; no EAE induction), matched for time from EAE induction.

Cat # Strain Termination Tissue Size Price
EZ-1111 C57BL/6 Time matched to EAE onset Spleen & spinal cord 1 mouse $261
EZ-1121 C57BL/6 Time matched to EAE peak Spleen & spinal cord 1 mouse $261
EZ-1131 C57BL/6 Time matched to EAE recovery Spleen & spinal cord 1 mouse $261
EZ-1211 SJL Time matched to EAE first wave onset Spleen & spinal cord 1 mouse $281
EZ-1221 SJL Time matched to EAE first wave peak Spleen & spinal cord 1 mouse $281
EZ-1231 SJL Time matched to EAE first wave remission Spleen & spinal cord 1 mouse $281
EZ-1241 SJL Time matched to EAE relapse onset Spleen & spinal cord 1 mouse $281
EZ-1251 SJL Time matched to EAE relapse peak Spleen & spinal cord 1 mouse $281
EZ-1261 SJL Time matched to EAE relapse recovery Spleen & spinal cord 1 mouse $281

(See also EAE tissue.)

Fresh cells from mice immunized with adjuvant only (control kit; no EAE induction). Termination at age 8 to 18 weeks.

Cat #StrainAntigenProductSizePrice(first mouse)Price (eachadd"l mouse)
CF-1101 C57BL/6 CFA only Spleen cells 1 mouse $161 $130
CF-1102 C57BL/6 CFA only Lymph node cells 1 mouse $161 $130
CF-1103 C57BL/6 CFA only Spleen cells and lymph node cells 1 mouse $205 $174
CF-2101 SJL CFA only Spleen cells 1 mouse $181 $150
CF-2102 SJL CFA only Lymph node cells 1 mouse $181 $150
CF-2103 SJL CFA only Spleen cells and lymph node cells 1 mouse $225 $194

Naïve tissue, cells, and serum

Snap frozen tissue from naïve mice (no immunization or disease induction).

Cat # Strain Termination Tissue Size Price
CZ-0100 DBA/1 Age 8 to 18 weeks Hind paw, spleen, serum Half mouse $94
CZ-0101 DBA/1 Age 8 to 18 weeks Hind paw, spleen, serum Half mouse $89
EZ-1100 C57BL/6 Age 8 to 18 weeks Spleen & spinal cord 1 mouse $110
EZ-1200 SJL Age 8 to 18 weeks Spleen & spinal cord 1 mouse $130

Fresh cells from naïve mice (no immunization or disease induction). Termination at age 8 to 18 weeks.

Cat #StrainAntigenProductSizePrice(first mouse)Price (eachadd"l mouse)
CF-1201 C57BL/6 none Spleen cells 1 mouse $138 $107
CF-1202 C57BL/6 none Lymph node cells 1 mouse $138 $107
CF-1203 C57BL/6 none Spleen cells and lymph node cells 1 mouse $182 $151
CF-2201 SJL none Spleen cells 1 mouse $158 $127
CF-2202 SJL none Lymph node cells 1 mouse $158 $127
CF-2203 SJL none Spleen cells and lymph node cells 1 mouse $202 $171

Frozen serum from naïve mice (no immunization or disease induction).

Cat # Strain Termination Tissue Size Price
CZ-0280 DBA/1 Age 8 to 18 weeks Serum 1 mL $406
CZ-5280 DBA/1 Age 8 to 18 weeks Serum 250 µL $149
SZ-0281 C57BL/6 Age 8 to 18 weeks Serum 1 mL $406
SZ-5281 C57BL/6 Age 8 to 18 weeks Serum 250 µL $149
SZ-0283 BALB/c Age 8 to 18 weeks Serum 1 mL $406
SZ-5283 BALB/c Age 8 to 18 weeks Serum 250 µL $149

Preparation

Mice 8 to 14 weeks of age are acclimated to our facility for at least 7 days.

For products from immunized mice (does not apply to products from naïve mice), mice are then immunized with adjuvant(s) only as follows:

Mouse strainHooke Control Kit(s)™ used for immunizationUsing protocol
DBA/1 (CIA controls)CK-0210 & CK-0211 (CFA & IFA emulsions)CIA Induction in DBA/1 Mice
C57BL/6 (EAE controls)CK-2110 (CFA emulsion)EAE Induction by Active Immunization in C57BL/6 Mice
SJL (EAE controls)CK-0120 (CFA emulsion)EAE Induction by Active Immunization in SJL Mice

Mice are euthanized at the timepoints given above.

Snap frozen tissue products

If serum is included, blood is collected immediately after termination and serum is isolated.

All tissue is snap frozen in liquid nitrogen and stored at -80 °C until shipment.

Fresh cell products

Cell suspensions are prepared and red blood cells in spleen are lysed.

Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.

Cells are shipped the same day for overnight delivery.

Frozen serum products

At termination blood is immediately collected, and serum is isolated.

Serum from multiple mice is pooled and stored at -80 °C until shipment.

Detailed contents - snap frozen tissue

Each product unit contains tissue and/or serum from a single mouse.

Each organ is supplied in a separate 1.5 mL cryogenic tube. Tubes are marked with a mouse ID, paw ID (left or right hind paw; if applicable), and tissue weight.

Typical tissue quantity:

  • Spinal cord 80 to 100 mg
  • Spleen 100 to 200 mg
  • Half spleen 40 to 80 mg
  • Hind paw 100 to 150 mg
  • Serum 100 µL

Detailed contents - fresh cells

Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.

Cells are supplied in 15 or 50 mL centrifuge tubes, in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).

Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cell will be pooled in one tube with one cell count.

(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)

The typical number of cells expected for naïve and immunized mice is as follows:

Cell typeCells/mouse(immunized)Cells/mouse(naïve)
Splenocytes100-200 x 10650-100 x 106
Lymph node cells10-30 x 1061-3 x 106

Detailed contents - frozen serum

Frozen serum, pooled from multiple mice, supplied in a 1.5 mL cryogenic tube on dry ice.

Storage & stability

Frozen tissue and serum - keep frozen until use. Storage at -80 °C is recommended.

Fresh cells - These ship cold on gel packs in insulated packaging. Use or freeze within 24 hours of shipment. For this reason fresh cells ship to US and Canadian addresses only, via FedEx overnight.

(We can supply frozen cells to international customers at an additional charge of $27/item. These ship on dry ice. Please specify "frozen cells" when ordering.)

Safety Data Sheets (SDS)

Frozen tissue and serum SDS (PDF)Fresh cell products SDS (PDF)

Related products

Tissue from mice with CIASerum from mice with CIATissue from mice with EAEControl and naïve tissue, cells, and serum

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