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sparQ Universal Library Quant Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Reactions are prepared by simply adding standard or diluted library sample. Optimized protocols with fast cycling condition are provided for both 10 μl or 20 μl reaction volumes. Following qPCR run, a standard curve is constructed which is then used to determine the concentrations of each unknown diluted library sample.
Results from sparQ and Roche KAPA Library Quant Kits were highly correlated. Concentrations of six different diluted libraries were determined using either the sparQ Universal Library Quant Kit on Quantabio Q qPCR cycler or the Roche KAPA Library Quantification Kit on Bio-Rad CFX following the manufacturer’s recommended protocol. Run times, including melt curves, were 40 minutes for sparQ and 80 minutes for KAPA.
sparQ Universal Library Quant Kits are manufactured with high lot-to-lot consistency. Concentrations of diluted libraries with low GC (library 1), high GC (library 2), or balanced GC-content (library 3) were determined using 3 different lots of sparQ DNA Standards. Each library sample was tested in quadruplicate reactions with each lot of sparQ DNA Standards. Standard deviations of average quantification values were all <0.13>0.13>
sparQ Universal Library Quant Kit is optinized for use on any qPCR Instrument. A tube of ROX is included in the kit for qPCR instruments requiring higher concentrations of the reference dye. This unique formulation minimizes pipetting steps and ensures precise qPCR results.
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