5PRIME Phase Lock Gel is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
5PRIME Phase Lock Gel (PLG) is a unique product that eliminates interphase-protein contamination during phenol extraction and ensures faster results with improved recoveries. PLG migrates to form a tight seal between the phases of an aqueous/organic extraction during centrifugation. The organic phase and the interphase materials are effectively trapped in or below the barrier, thus enabling complete and easy decanting or pipetting of the entire aqueous phase. The benefits are increased yields of up to 30%, increased protection from exposure to hazardous compounds, and no risk of interphase sample contamination.
PLG can be adapted to virtually any protocol requiring extraction of an aqueous sample with phenol and/or chloroform1. For convenience, PLG is provided predispensed into standard 2mL centrifuge tubes and comes in two varieties:
PLG Heavy
Aqueous phase: High density sample (for example, high salt content).
Organic phase: Standard mixtures of phenol, chloroform, isoamyl alcohol with a chloroform fraction of at least 60% (Phenol as the sole organic solvent is not compatible with PLG Heavy).
PLG Light
Aqueous phase: Low-density sample
Organic phase: Standard mixtures of phenol, chloroform, isoamyl alcohol.
PLG is inert and stable to mild heating, and it does not interfere with any of the standard nucleic acid restriction and modification enzymes. The reactions can be carried out in the presence of PLG at the appropriate temperature and then terminated and extracted by the addition of phenol or phenol/chloroform to the reaction tube. PLG can be present during the heating stage of the deactivation of enzymes (65°C for 10 min.) prior to the organic extraction.
Applications:
Determine which version of Phase Lock Gel suits your experiment conditions.
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