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Extracta DNA Prep is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
2 mm tail snips were extracted in 150 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 1.5 µL of each extract was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.
1 mm diameter mouse ear punches were extracted in 100 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 5.0 µL of each extract was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.
Extracta reduces time to results with a rapid, convenient protocol.
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