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QuantaBio/AccuStart II PCR Genotyping Kit/100 x 25 μL rxns/PCR_Genotyping

Features & Benefits

  • Simplified, completely reagent-based system requires minimal pipetting skill
  • Premixed electrophretic mobility loading dye reduces chances for post-PCR cross contamination
  • Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X)
  • High-yielding,ultrapuremodified Taq DNA polymerase delivers robust, reliable duplex assay performance
  • Stringent,ultrapureantibody hotstart ensures sensitive and specific target amplification

AccuStart II PCR Genotyping Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It combines a rapid, 2-component DNA extraction reagent with a user-friendly 2X concentrated PCR SuperMix with loading dye for seamless gel electrophoresis analysis. qPCR-grade genomic DNA template is obtained with minimal extraction volumes (≤ 100uL) and can be carried out in ≤ 30-minutes on a standard PCR thermal cycler.

Performance Data

Accustart II vs KAPA: Mouse tail snips

Accustart II vs KAPA: Mouse tail snips

AccuStart II Results more specific

KAPA PCR mix requires more optimization to reduce non-specific amplification products in multiplex PCR reactions. Lanes: 1 = mouse 1, ZPBP1. 2 = mouse 2, ZPBP1. 3 = mouse 1, ZPBP1/p53. 4 = mouse 2, ZPBP1/p53. 5 = mouse 1, p53. 6 = mouse 2, p53

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