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immusmol/Dopamine ELISA kit - Urine-Plasma - Fast Track/BA-E-6300

Product informationKey stepsReferencesTechnical supportReviews

Product overview

Product name

Dopamine ELISA kit - Urine/Plasma - Fast-Track

DescriptionIn vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Dopamine levels in plasma and urine samples.
LabelsIVD, CE
Format96-well plate
Samples10 µl urine, 300 µl plasma
Cross specie-reactivityReact with all species
Standard range0 / 4.5 - 2 000 ng/ml
Sensitivity

4.5 ng/ml urine, 25 pg/ml plasma

Specificity

No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.

Assay time4 hours
StorageStore at 2-8°C for up to 6 months.
DatasheetsInstructions for useMaterial safety datasheet
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Key steps

Sample collection & storage

Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C.

Sample preparation

Sample preparation, extractiion and acylation (65min)

ELISADopamine antiserum incubation (2H), revelation and read steps (1h).
Detailed protocolDownload instructions for use
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References

  • Gaber EW & El-Attar HA. The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy. British Journal of Diabetes & Vascular Disease May/June 2013 vol. 13 no. 3 130-137
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