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indoor biotechnologies/Natural Bla g 2 (NA-BG2-1)/1/NA-BG2-1

Allergen:Bla g 2 (Blattella germanica allergen 2), German cockroach
Unit:250µg
Source: B. germanica cockroach frass
Mol. Wt:~36 kD
Purification:

Purified from cockroach extract by monoclonal antibody affinity chromatography. Purity by silver stained SDS-PAGE > 95%.

Concentration:See package insert.
Formulation:

Preservative-free and carrier-free in phosphate buffered saline,pH 7.4. Sterile filtered.

Storage:Store at -20ºC
Notes:(1) Natural Bla g 2 purifies as a 36 kD monomer and 72 kD dimer.
PDB:1YG9, 2NRGX-ray crystal structures
Product Resources:Natural Bla g 2 Certificate of Analysis
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.
References:
  1. Pollart SM, Mullins DE, Vailes LD, Hayden ML, Platts-Mills TAE, Sutherland WM, Chapman MD. Identification, quantitation, and purification of cockroach allergens using monoclonal antibodies. J Allergy Clin Immunol 1991;87:511-21.
  2. Arruda LK, Vailes LD, Mann BJ, Shannon J, Fox JW, Vedvick TS, Hayden ML, Chapman MD. Molecular Cloning of a major cockroach (Blatella germanica) allergen, Bla g 2: Sequence homology to the aspartic proteases. J Biol Chem 1995;270:19563-8.
  3. Pomés A, Chapman MD, Vailes LD, Blundell TL, Venugopal D. Cockroach allergen Bla g 2: Structure function and implication for allergic sensitization. Amer J Resp Crit Care Med 2002;165:391-7.
  4. Wünschmann S, Gustchina A, Chapman MD, Pomés A. Cockroach allergen Bla g 2: An unusual aspartic proteinase. J Allergy Clin Immunol 2005;116:140-5.
  5. Gustchina A, Li M, Wünschmann S, Chapman MD, Pomés A, Wodawer A. Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition. J Mol Biol 2005;348:433-44.
  6. Li Mi, Gusrchina A, Alexandratos J, Wlodawer A, Wünschmann S, Kepley CL, Chapman MD, Pomés A. Crystal Structure of a dimerized cockroach allergen Bla g 2 complexed with a monoclonal antibody. JBC 2008, in press.
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