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ELISA 2.0 kit for measuring Oryctolagus cuniculus allergen, Ory c 3. | |||
| Antibodies: | pAb/pAb | ||
| Standard Range: | 20-0.04 ng/mL | ||
| Limit of Detection: | 0.08ng/mL | ||
| Background: | OD<0.08 at 450nm | ||
| Coefficient of Determination: | R-squared>0.98 | ||
| Contents: | Plate: Pre-coated with anti-Ory c 3 purified polyclonal rabbit antiserum.Vial 1: (white top) Ory c 3 allergen standardConcentration: 200ng/mlVial 2: (brown) Biotinylated purified polyclonal rabbit anti-Ory c 3 antibody.Vial 3: (blue top) Streptavidin-peroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||
| Storage: | Store at 2-8°C | ||
| Expiry: | 6 months from date of receipt | ||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||
| Notes: | The allergen standard is recommended for immunoassay calibration purposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||
| Product Resources: | EPC-OC3-5 Certificate of Analysis Validation Performance Data | ||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||
| Certificate of Analysis | |||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withRabbit polyclonal antiserum and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||
| Coating Antibody: | Rabbit polyclonal antiserum | ||
| Immunogen: | Ory c 3 | ||
| Isotype: | Multiple | ||
| Specificity: | Binds to an epitope on rabbit Oryctolagus cuniculus allergen, Ory c 3. | ||
| Purification: | Produced in vivo and purified using Affinity chromatography. Single heavy and light chain bands on SDS-PAGE. | ||
| Certificate of Analysis | |||
| Detection Antibody: | Rabbit polyclonal antiserum | ||
| Immunogen: | Ory c 3 | ||
| Isotype: | Multiple | ||
| Specificity: | Binds to an epitope on rabbitOryctolagus cuniculus allergen, Ory c 3. | ||
| Purification: | Produced in vivo and purified using Affinity chromatography. Single heavy and light chain bands on SDS-PAGE. | ||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | ||
| Certificate of Analysis | |||
| Allergen Standard: | Recombinant Ory c 3 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | ||
| Concentration: | 200ng/mL (based on amino acid analysis) | ||
References: | |||
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn