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Molecular Devices/FLIPR Potassium Assay Kit/Evaluation Kit - PN: R8330/4472462147

The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels. In this assay, a novel, highly-sensitive Tl+ indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl+ conducted through potassium channels. The intensity of the Tl+ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio.

  • Functional measurement of potassium channel activity in a cell-based assay
  • Homogenous no-wash protocol reduces well-to-well variation and simplifies the workflow
  • Expanded signal window compared to non-homogenous assay
  • Rapid procedure with less hands‐on time

 

 

Available Configurations:

 ConfigurationDescriptionPart Number
Explorer Kit
  • (10) Vials of Component A
  • (1) Bottle of Component B* assay buffer HBSS + Ca2+, Mg2+ and 20 mM HEPES, pH 7.4
  • (10) Vials of Component C
  • (1) Bottle of 5X chloride-free assay buffer
  • (1) Bottle of potassium sulfate solutin (200 mM)
  • (1) Bottle of thallium sulfate solution (50 mM)
Each kit contains enough material for ten (10) 96-well or 384-well microplates. Each vial is sufficient for one (1) microplate experiment.		(R8222)
Bulk Kit
  • (10) Vials of Component A
  • (10) Vials of Component C
  • (1) Bottle of 5X chloride-free assay buffer
  • (1) Bottle of potassium sulfate solutin (200 mM)
  • (1) Bottle of thallium sulfate solution (50 mM)
Each kit contains enough material for one hundred (100) 96-well or 384-well microplates. Each vial is sufficient for ten (10) microplate experiments.		(R8223)
Evaluation Kit
  • (2) Vials of Component A
  • (1) Bottle of Component B* assay buffer HBSS + Ca2+, Mg2+ and 20 mM HEPES, pH 7.4
  • (2) Vials of Component C
  • (1) Bottle of 5X chloride-free assay buffer
  • (1) Bottle of potassium sulfate solutin (200 mM)
  • (1) Bottle of thallium sulfate solution (50 mM)

Each kit contains enough material for two (2) 96-well or 384-well microplates. Each vial is sufficient for one (1) microplate experiment.

(R8330)
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