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Discovery Antibodies/Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody/50 µl/AAR-011-F-50 µl

Cat#:AAR-011-F
AlternativeNameAT1Receptor,AT1R,AGTR1
  • LyophilizedPowder
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • PeptideNSSTEDGIKRIQDDC,correspondingtoaminoacidresidues4-18ofhumanAT1 receptor(Accession P30556).Extracellular,N-terminus.
        Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
    Accession(Uniprot)NumberP30556
    GeneID185
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse,rat,rabbit,dog-identical.
    RRIDAB_2756538.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    LabelFluoresceinisothiocyanate(FITC).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-011)anddirectflowcytometry(labeledantibody).
    Applications:fc
    Mayalsoworkin:ic*,lci*
      • Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
        CellsurfacedetectionofAT1 receptorinliveintacthumanTHP-1monocyticleukemiacells:
        ___Cellsalone.
        ___Cells+RabbitIgGisotypecontrol-FITC.
        ___Cells+AngiotensinII ReceptorType-1(extracellular)-FITC Antibody(#AAR-011-F),(5µg).
        Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
        CellsurfacedetectionofAT1 receptorinliveintacthumanJurkatT-cellleukemiacells:
        ___Cellsalone.
        ___Cells+RabbitIgGisotypecontrol-FITC.
        ___Cells+AngiotensinII ReceptorType-1(extracellular)-FITCAntibody(#AAR-011-F),(5µg).
    References
    • 1.deGasparo,M.etal.(2000)Pharmacol.Rev.52,415.
    • 2.Balakumar,P.andJagadeesh,G.(2014)J.Mol.Endocrinol.53,R71.
    • 3.Graus-Nunes,F.etal.(2017)Mol.Cell.Endocrinol.439,54.

      • TheBIOLOGicalfunctionsof angiotensinII aremediatedby AT1 and AT2 receptors.BothbelongtotheG-proteincoupledreceptorsuperfamilyandarecomprisedofsevenhydrophobictransmembranesegmentsformingαhelicesinthelipidbilayerofthecellmembrane,anextracellularN-terminusandanintracellularN-terminus1.

        Twohighlyhomologousisoformsofthereceptor,AT1A andAT1Bhavebeenidentifiedinrodents.TheAT1A receptoraccountsfor90%ofthetotalbinding,andispredominantinthekidney,vascularsmoothmusclecells,heart,liver,andinsomeareasofthebrain,whiletheAT1B receptorisfoundpredominantlyinthepituitaryandadrenalglands,placenta,lung,andbrain.Theseisoformsarepharmacologicallyindistinguishable andarebothselectivelyantagonizedby losartan.

        ThroughAT1receptor,angiotensinIIstimulatesmultiplesignalingpathways,cross-talkswithseveraltyrosinekinases,andtrans-activatesgrowthfactorreceptors.PhosphorylationofthereceptorbyGPCRkinasesterminatesreceptoractivationandpromotesβ-arrestinrecruitment.β-arrestin-scaffoldedsignalingmediates‘secondarysignaling’thatinvolvesmultiplekinasesthatlinktocellprotectivedownstreamsignalingmolecules2.

        Inamodelofdiet-inducedobesemice,AT1receptorinhibitiondemonstratesbeneficialpleiotropiceffects.Micereceivingthetreatmentexhibitenhancedpancreaticduodenalhomeobox1(PDX1)and GLP-1 pancreaticisletexpression.PDX1isletsareessentialfortheexpressionofglucokinaseand GLUT2.GreaterGLP-1levels,isletvascularizationandreducedapoptosisandmacrophageinfiltrationarealsoobserved3.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowder
    Lastupdate:29/07/2019

    Anti-AngiotensinIIReceptorType-1(extracellular)Antibody(#AAR-011)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,immunocytochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeAT1 receptorfromrat,mouse,andhumansamples.

    Anti-AngiotensinIIReceptorType-1(extracellular)-FITCAntibody(#AAR-011-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.

    Forresearchpurposesonly,notforhumanuse
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