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Discovery Antibodies/Anti-IP3 Receptor-2 (ITPR2) Antibody/25 µl/ACC-116-25 µl

Cat#:AER-051-F
AlternativeNameEMR1hormonereceptor, EGF-likemodule-containingmucin-likehormonereceptor-like1,AdhesionGprotein-coupledreceptorE1,TM7LN3,F4/80,GPF480
  • LyophilizedPowder
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • PeptideCKQGFLSSNGQNHFK,correspondingtoaminoacidresidues58-72ofhumanEMR1(Accession Q14246).Extracellular,N-terminus.
        Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    Accession(Uniprot)NumberQ14246
    GeneID2015
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse,Rat–11/14aminoacidresiduesidentical.
    RRIDAB_2756589.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    LabelFluoresceinisothiocyanate(FITC).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AER-051),anddirectflowcytometry(labeledantibody).
    Applications:fc
    Mayalsoworkin:ic*,lci*
      • Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
        CellsurfacedetectionofEMR1inliveintactmouseJ774macrophagecells:
        ___ Cells.
        ___ Cells+rabbitIgGisotypecontrol-FITC.
        ___ Cells+Anti-EMR1(ADGRE1)(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
        Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
        CellsurfacedetectionofEMR1inliveintacthumanTHP-1monocyticleukemiacells:
        ___ Cells.
        ___ Cells+rabbitIgGisotypecontrol-FITC.
        ___ Cells+Anti-EMR1(ADGRE1)(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
    References
    • 1.Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
    • 2.McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
    • 3.Araç,D.etal.(2012)EMBOJ.31,1364.

      • Humanepidermalgrowthfactor(EGF)–likemodulecontainingmucin-likehormonereceptor1(EMR1)isasurfacereceptorwithunknownfunctionthatbelongstotheadhesionfamilyoftheGprotein-coupledreceptors(GPCR).ThisreceptorisencodedbytheADGRE1andlikeallGPCRs,containsseventransmembranedomains,anintracellularC-terminusandanextracellularN-terminus.TheNH2domainhassixEGF-likedomains.

        AdhesionGPCRssharealongN-terminalextracellularsequencescomprisingmultipledomains,separatedfromthetransmembranesegmentsbyaserine/threonine-richdomain,afeaturereminiscentofmucin-like,single-span,integralmembraneglycoproteinswithadhesiveproperties1,2.

        EMR1proteinisahomologofF4/80,amurineproteinthatiswidelybeingusedasaMarkerofmurinemacrophagepopulations1.

        HumanEMR1isexclusivelyexpressedonmatureeosinophilsinthebloodandbonemarrow andinnasalpolyps.Thus,EMR1is consideredtobeapotentialtherapeutictargetforthetreatmentofeosinophilicdisorders1,3.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowder
    Lastupdate:16/09/2019

    Anti-EMR1(ADGRE1)(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.

    Anti-EMR1(ADGRE1)(extracellular)–FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

    Forresearchpurposesonly,notforhumanuse
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