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| ProductName | Wilm’sTumorSuppressor1,WT-1(+KTS) |
| Size | 5,000U |
| Description | WT-1,theproductofWilms’tumorsuppressorgeneWt1,isanuclearproteinwithstructuralmotifscharacteristicoftranscriptionfactors,includingfourC-terminalzincfingers.Whiledifferentpre-mRNAprocessingcouldresultin16isoformsoftheprotein,theinclusionorexclusionofexon5andthreeaminoacids(KTS)betweenzincfingers3and4largelyaffectstheactivityoftheWT1protein(1-3).Suchacomplexpost-transcriptionalregulation,particularlyinsplicing,mayrepresentamajorregulatorymechanismfortumOrigenesisoftheWilms’tumor.Withtheinclusionofexon5,WT1(KTS+)bindstobothDNAandRNAandisRNasebutnotDNasesensitive(4).Thisformalsoco-localizeswithsplicingfactorsinaspecklednuclearparticle,suggestingthattheWT1proteinmayfunctionasbothatranscriptionfactorandasplicingregulator(5,6) |
| Applications | invitrofunctionstudiesincluding
|
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitistheamountsufficientforagelmobilityshiftassayina20µlreaction.50unitsaresufficientforreconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 7490 |
| Accession# | NM_024426 |
| GeneName | Wilmstumor1 |
| GeneSymbol | WT1 |
| GeneSynonyms | GUD;AWT1;WAGR;WT33;NPHS4;WIT-2;EWS-WT1 |
| MW | 54.6kDa |
| ProteinSequence | 1MQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRG 61ASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLG 121GPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPF 181GPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFP 241NHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQ 301LECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHG 361VFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKP 421YQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEK 481PFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL |
| StorageBuffer | 20mMTris-Cl(pH8.0),50%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Haber,DA.etal.,(1991)Proc.Natl.Acad.Sci.USA88,9618-9622;(1993)Science262,2057-2059 2.Kreidberg,JA.etal.,(1993)Cell74,679-691 3.Larsson,SH.etal.,(1995)Cell81,391-401 4.Caricasole,A.,etal.,(1996)Proc.Natl.Acad.Sci.USA,93,7562-7566 5.Little,M.etal.,(1997)Hum.Mutat.9,209-225 6.Englert,C.etal.,(1995)EMBOJ.14,4662-4675 |
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