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| ProductName | c-jun(protooncogene) |
| Size | 5,000U |
| Description | ThetranscriptionfactorAP-1(activatorprotein-1)isinvolvedincellularproliferation,transformationanddeath.AP-1andnuclearfactorB(NF-kB)canbespecificallytargetedtopreventcancerinductioninmousemodels.AP-1canbeproducedby18differentdimericcombinationsofproteinsfromtheJun(c-Jun,JunBandJunD)andFos(c-Fos,FosB,Fra-1andFra-2)families,includingJunhomodimersandJun–Fosheterodimers(1–4).TheJunandFosproteinscontainabasic-regionleucinezipper(bZIP)domain,andarecapableofbindingtootherbZIPproteinsincludingthosefromtheATF,MAF,CNCandC/EBP(CCAAT/enhancer-bindingprotein)subfamilies(5])Jun–JunandJun–Fosdimersbindwithhighestaffinitytothe12-O-tetradecanoylphorbol-13-acetate(TPA)responseelement(TRE)[TGA(C/G)TCA](6),althoughmanyother`AP-1-likesites`havebeenreported.Bindingtoanyofthesesitescanbetissue-specific,oraffectedbyneighboringsequences,anddependentuponinteractionswithothertranscriptionfactorsorcofactors.JunandFosproteinscanalsodimerizewithotherbZIPproteins,allowingthemtotargetotherDNAbindingsites,suchasthecAMPresponseelement(CRE),theantioxidantresponseelements(ARE),andhalf-sitescomposedofhalfofaTREsiteandhalfofaMAF-orCNC-bindingsite.Inaddition,AP-1proteinscaninteractwithotherproteins,includingthep65subunitofNF-kB,CBP(CRE-binding-protein-bindingprotein)(p300),SMAD-3and-4,andtheretinoblastomaprotein(see5foramorecompletelist),furtherincreasingthecombinatorialpotentialofJunandFosproteins.AP-1regulatesavarietyofcellularprocesses,includingproliferation,differentiationandapoptosis,andcontributestobothbasalandstimulus-activatedgeneexpression.Itisactivatedbygrowthfactors,hormones,stress,cytokines,ROSandultravioletrADIationActivationoccursbothtranscriptionallyandpost-translationally,andissignaledpredominantlythroughthemitogen-activatedproteinkinase(MAPK)cascade(7).ThecombinatorialdiversityofAP-1proteinsandotherinteractingfactorsappearstoinfluencehowspecificcelltypesrespondtoastimulus.Thegrowth-promotingactivityofc-Junismediatedbyrepressionoftumorsuppressors,aswellasup-regulationofpositivecellcycleregulators.Mostly,c-Junisapositiveregulatorofcellproliferation,whereasJunBhastheconverseeffect(8). |
| Applications | DNAandprotein-proteininteractionassays |
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 3725 |
| Accession# | NM_002228 |
| GeneName | junproto-oncogene |
| GeneSymbol | JUN |
| GeneSynonyms | AP1;AP-1;c-Jun |
| MW | 37.5kDa. |
| ProteinSequence | MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDL LTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAE LHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGA LSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETP PLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANM LREQVAQLKQKVMNHVNSGCQLMLTQQLQTF |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.CurranT.andFranzaB.R.Jr(1988)Cell55,395-397 2.RauscherF.J.D.etal.(1988)Science,240,1010-1016 3.RauscherF.J.D.etal.(1988)Cell52,471-480 4.WagnerE.F.(2001)Oncogene20,2333-2497 5.ChinenovY.andKerppolaT.K.(2001)Oncogene20,2438-2452 6.AngelP.etal.(1987)Cell49,729-739 |
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