飞思卡尔和Monebo携手推出创新的心脏监视平台

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Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103)

Even more from less, for DNA

Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.

Get more of what you need, with the highest library yields Use to generate high quality librariesfrom a broad range of input amounts, from 500 pg to 1 g Prepare libraries from ALL your samples, including GC-rich DNAand FFPE DNA samples Improve yields and quality for target enrichment applications Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats

Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers. This kit is available withand withoutsample purification beads.

The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities.The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.

Now also available with optional SPRIselect beadsfor clean-up and size-selection steps.
Advantages Get more of what you need, with the highest library yields Use to generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples Improve yields and quality for target enrichmentapplications Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats To learn more about how NEBNext Ultra II addresses lower input amounts and challenging sample types, download our technical note.

For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
NEBNext Ultra II produces the highest library yields and conversion rates from a broad range of input amounts
A: Libraries were prepared from Human NA19240 genomic DNA using the input amounts and numbers of PCR cycles shown. Manufacturers recommendations were followed, with the exception that size selection was omitted.
B: Libraries were prepared from Human NA19240 genomic DNA using the input amounts and library prep kits shown without an amplification step, following manufacturers recommendations. qPCR was used to quantitate adaptor-ligated molecules, and quantitation values were then normalized to the conversion rate for Ultra II. The Ultra II Kit produces the highest rate of conversion to adaptor-ligated molecules, for a broad range of input amounts.


View additional data on library yield
NEBNext Ultra II provides uniform GC coverage for microbial genomic DNA over a broad range of GC composition and input amounts
Libraries were made using 500 pg, 1 ng and 100 ng of the genomic DNAs shown and the Ultra II DNA Library Prep Kit and sequenced on an Illumina MiSeq . Reads were mapped using Bowtie 2.2.4 and GC coverage information was calculated using Picards CollectGCBiasMetrics (v1.117). Expected normalized coverage of 1.0 is indicated by the horizontal grey line, the number of 100 bp regions at each GC% is indicated by the vertical grey bars, and the colored lines represent the normalized coverage for each library. View additional data on library quality
NEBNext Ultra II provides superior yields in PCR-free workflows
Libraries were generated from 100 ng of Human NA19240 genomic DNA using the library prep kits shown, following manufacturers recommendations, and with no amplification step. Library yields were determined by qPCR using the NEBNext Library Quant Kit for Illumina. The NEBNext Ultra II Kit produces the highest yields.

NEBNext Ultra II libraries provide the highest quality sequence data in PCR-free workflows

PCR-free libraries were generated from 100 ng of Human NA19240 genomic DNA using library prep kits shown, following manufacturers recommendations, and with no amplification step. Libraries were sequenced on the Illumina NextSeq 500. Reads were mapped to the GRCh37 reference using Bowtie 2.2.4.This data illustrates that NEBNext Ultra II DNA Library Prep Kit enables high quality sequence data in PCR-free workflows, even with low input amounts.
% Mapped:The percentage of reads mapped to human GRCh37 reference.
% Duplication:The percentage of mapped sequence that is marked as duplicate.
% Chimeras:The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes.
NEBNext Ultra II enables construction of high quality libraries from FFPE DNA samples

Libraries were prepared from 17 30 ng of human DNA extracted from the FFPE tissue samples listed, amplified using 10 cycles of PCR and sequenced on the Illumina MiSeq.
Reads were mapped to the GRCh37 reference genome using Bowtie 2.2.4.
% Mapped:The percentage of reads mapped to Human GRCh37 reference.
% Mapped in Pairs:The percentage of reads whose mate pair was also aligned to the reference.
% Duplication:The percentage of mapped sequence that is marked as duplicate.
% Chimeras:The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes.
This data illustrates that the NEBNext Ultra II DNA Library Prep Kit for Illumina enables high quality sequence data, even with low input amounts of FFPE DNA.
View additional data from FFPE DNA samples

NEBNext Ultra II provides superior yields for target enrichment applications.

For Nimblegen enrichment, libraries were prepared with 100 ng of Human HG02922 genomic DNA, using NEBNext Ultra II with the NEBNext Adaptor and index primers, or the Kapa Library Preparation Kit Illumina Platforms with the SeqCap Adapter Kit (Roche). Target enrichment was performed with NimbleGen SeqCap Human Exome v3.
For SureSelectXT enrichment, libraries were prepared with 200 ng of Human HG02922 genomic DNA, using NEBNext Ultra II or Kapa Hyper with the NEBNext Adaptor and index primers, or the SureSelectXT Reagent Kit, Illumina (ILM) platforms with Herculase II Fusion DNA Polymerase. Target enrichment was performed with SureSelect Human All Exon V6.
Manufacturers recommendations were followed, including use of the recommended number of PCR cycles for pre- and post-capture. NEBNext Ultra II libraries resulted in the highest post-capture yields for both enrichment workflows.

View additional data on target enrichment

Lot Control The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page Kit Components

The following reagents are supplied with this product:

0.1X TE (1 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) DNA LoBind Tubes (Eppendorf #022431021) NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600, #E6609, #E7710, or #E7730) Magnetic rack or plate (e.g., NEBNext Magnetic Separation Rack (NEB #S1515S), Alpaqua 96S Super Magnet Plate (#A001322), or equivalent) PCR Machine

For NEB #E7645 only:

SPRIselect Reagent Kit (Beckman Coulter, Inc. #B23317) or AMPure XP Beads (Beckman Coulter, Inc. #A63881) Optional: 10 mM Tris-HCl, pH 8.0 or Tris/NaCl, pH 8.0 (10 mM Tris, 10 mM NaCl)
(for adaptor dilution of DNA input 100 ng)
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualE7103-E7645
Gudbjartsson, D.F., et al. (2020) Early spread of SARS-Cov-2 in the Icelandic population medRxiv; DOI: https://doi.org/10.1101/2020.03.26.20044446 DNA Pipelines R D, et al. (2020) COVID-19 ARTIC v3 Illumina library construction and sequencing protocol V.3 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bgq3jvyn
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] E7645S_L_v1
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New England Biolabs (NEB) is committed to practicing ethical science we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more. Licenses

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This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.

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　飞思卡尔半导体和Monebo Technologies近日结成战略伙伴，为使用心电图（ECG）技术的医药设备提供综合平台。片上ECG解决方案将Monebo的Kinetic ECG软件和飞思卡尔嵌入式处理技术集于一身，使医疗设备生产商能够开发出使用简便的ECG监视工具。
ECG监视器是医疗保健机构用来确定心脏状况、监视病人健康的重要工具。据世界卫生组织的数据，心血管病已经成为造成全球人口死亡的主要因素。2005年，全球大约有1750万人死于心血管疾病，占全球总死亡人数的30％。在这些死亡人口中，760万人死于心脏病发作，570万人死于中风。 到2015年，每年将会有约2000万人死于心血管疾病，死亡的主要原因都是心脏病发作和中风。而他们中的很多人可能是在事先没有任何心血管疾病症状的情况下。
“受多种心血管疾病影响的人通常并没有接受治疗，或者是因为他们没有意识到他们已经得了这类疾病，或他们对传统治疗方案有一些误解。”飞思卡尔全球医疗应用专家Jose Fernandez Villasenor博士表示，“而有了像飞思卡尔和Monebo这样的公司的突破性硅和软件技术，使得用关键的和人们普遍接受的ECG测试进行心脏病人的监视变得更轻松、更经济高效。”
为了帮助医疗专业人士评估心脏参数，Monebo Technologies开发了支持ECG波形的信号处理和解释的Kinetic ECG算法。该算法能够为捕获到的最多16个导联的ECG数据提供非常精确的QRS(Q波形、R波形和S波形)检测和特征提取、心跳分类、间歇测量和心律解释。
Monebo的Kinetic软件运行在各种飞思卡尔微处理器（MPU）和微控制器（MCU）上。这些处理平台选项使开发人员能够根据他们的性能、操作功率、集成和系统成本需求，自由选择ECG应用。
“依托我们独特的软件算法和飞思卡尔处理平台，我们拥有为临床医生提供详细信息的技术，使医生能够远程监视心脏病人，并且从任何设备上分析和解释ECG数据，包括那些用于药物和临床试验的数据。” Monebo Technologies总裁兼首席执行官 Dale Misczynski表示。
加快医药设备设计人员产品面市
当今的医疗设备设计人员面临着如何设计更尖端的产品，同时将这些产品更快地推向市场的挑战。Kinetic算法和飞思卡尔处理器的结合，使设计人员能够在设计中增加先进的ECG监视功能，同时将产品更快推向市场。飞思卡尔嵌入式MPU和MCU包含必要的集成，在下一代医疗设备中增加了创新技术。由于Kinetic算法通过了美国食品和药品管理局（FDA）510(k) 认证，因此简化了最终设备监管备案。
Monebo 软件可以与飞思卡尔的MCU与嵌入式MPU系列结合使用，如8位HCS08和16位数字信号控制器（DSC）、32位ColdFire、 PowerQUICC 和i.MX器件。这一硬件/软件联合解决方案的整体优势包括：
加快产品面市——通过简化PDA验证，缩短开发时间；
可扩展性——医疗设备设计人员可以根据飞思卡尔嵌入式处理器和Monebo软件的独特功能开发最适宜的设计。
更低的开发成本——片上ECG解决方案经过全面的测试和验证，为医疗设计人员提供“一站式商店”方法。
“飞思卡尔认为，如果消费者能够拥有一些他们日常生活息息相关的医疗设备，他们就能更有效地监视自己的健康状况。” 飞思卡尔高级副总裁兼首席销售和营销官Henri Richard表示，“通过缩短产品上市时间、提供一流硬件、降低系统成本，飞思卡尔技术将进一步拓宽ECG医疗设备市场。最终，片上ECG解决方案将会带来新一代ECG产品，帮助提高深受心血管疾病之苦的亿万人的生活质量。”
片上ECG解决方案非常适合于大量应用技术，如：
事件记录：自动触发告警，警告心脏问题和呼叫中心决策支持
病人临床监视：通过医生的即时信息接入，进行实时监视病人
配药：临床试验的间歇测量 ，或者监视和调整病人药物剂量
辅助心脏恢复的监视器
家庭医疗：在家中监视病人的便携式设备
医疗和健身：监视心脏健康和优化试验的设备
即于2008年6月16-19日在佛罗里达奥兰多举行的飞思卡尔技术论坛上，Monebo和飞思卡尔将演示片上ECG解决方案。

Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities(500 picograms to 1 mic...