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NEB/OneTaq® Quick-Load® 2X Master Mix with GC Buffer/M0487S/100 reactions (50 μl vol)188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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产品资料

NEB/OneTaq® Quick-Load® 2X Master Mix with GC Buffer/M0487S/100 reactions (50 μl vol)

Description:

OneTaq®Quick-Load®2XMasterMixwithGCBufferisanoptimized,ready-to-useblendofTaqandDeepVentR™DNAPolymerasesideallysuitedtoPCRapplicationsfromGC-richtemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Theconvenientquick-loadmastermixformulationcontainsdNTPs,MgSO4,buffercomponentsandstABIlizersaswellastwocommonlyusedtrackingdyesforDNAgels.Ona1%agarosegelin1XTBE,XyleneCyanolFFmigratesat~4kbandTartrazinemigratesat~10bp.Bothdyesarepresentinconcentrationsthatdonotmaskco-migratingDNAbands.

AmplificationofaselectionofsequenceswithvaryingGCcontentfromhumangenomicDNAusingOneTaqDNAPolymerase.AmpliconsizesareindicatednexttogelandGCcontentisindicatedabovegel.MarkerMisthe1kbDNALadder(NEB#N3232).
  

ProductSource

AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andan E.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.

ReagentsSupplied

Thefollowingreagentsaresuppliedwiththisproduct:

Storeat(°C)Concentration
OneTaq HighGCEnhancer50%

Notes:

ProductspecificationsforindividualcomponentsintheOneTaqQuick-Load2XMasterMixwithGCBufferareavailableseparately.OneTaqQuick-Load2XMasterMixwithGCBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqQuick-Load2XMasterMixwithGCBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.OneTaqHighGCEnhancer(1X):10mMTris-HCl25%DMSO25%GlycerolpH9.2@25°C
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