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Bpsbioscience/HDAC11, GSTtag/50011/50 µg
Species:Human
HostSpecies:Sf9insectcells
MW:66kDa
Genbank#:NM_024827
Tag(s):N-terminalGST-tag
a.a:fulllength
Description:
HumanHDAC11,fulllength,GenBankAccessionNo.NM_024827,withN-terminalGST-tag,MW=66kDa,expressedinbaculovirusexpressionsystem.
UniProt
Q96DB2
Synonym(s):
histonedeacetylase11,HDAC-11
SpecificActivity:
≥3.7pmol/min/µg.
AssayConditions:
25mMTrisHCl,pH8.0,137mMNaCl,2.7mMKCl,1mMMgCl2,and0.1mg/mlBSA,20µMBPSHDACclass2asubstrate(Catalog#50040),andHDAC11(15.6-2000ng).Incubationcondition:35minat37°C,followedbyHDACdeveloper(Catalog#50030)for15minatroomtemperature.Fluorescenceintensityismeasuredatex360/em460.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glycerol
Format:
Aqueousbuffersolution
Storage/Stability:

>6monthsat-80°C.

Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):

Strausberg,R.L.etal.,Proc.Natl.Acad.Sci.U.S.A.99(26),16899-16903(2002).

ApplicationReference(s):
1. TargetingHistoneDemethylasesinMYC-DrivenNeuroblastomaswithCiclopirox(2017)
2. Reassessingtheeffectsofhistonedeacetylaseinhibitorsonhippocampalmemoryandcognitiveaging(2014)

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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