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Bpsbioscience/PDE3A (Mouse), GST-Tag/60036/5 µg

Species:Mouse
HostSpecies:Sf9insectcells
MW:100kDa
Genbank#:NM_018779
Tag(s):N-terminalGST-tag
a.a:484-1141
Description:
MousePDE3A(GenBankAccessionNo.NM_018779),aminoacids484-1141end),withN-terminalGSTtag,MW=100kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
PDE3A,phosphodiesterase3A,PDE3
SpecificActivity:
≥280pmol/min/µg
AssayConditions:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3′,5′-cAMPto5′-AMPperminat37°C.Assaycondition:10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,andserialdilutionsofMousePDE3Aat37ºCfor20min.Quantifiedby5′nucleotidasecleavingthe5′-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween20,20%glycerol,18mMGlutathione
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Hung,S.H.,etal.J.Biol.Chem.281(39),29236-29244(2006)

2.Hambleton,R.,etal.J.Biol.Chem.280(47),39168-39174(2005)

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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