Bpsbioscience/NET – CHOK1 Recombinant Cell Line/60557/2 vials188bio精品生物—专注于实验室精品爆款的电商平台

Background:

NETisamonoaminetransporterresponsIBLeforthesodium-chloride(Na+/Cl−)-dependentreuptakeofextracellularnorepinephrine(NE),alsoknownasnoradrenaline.NETcanalsoreuptakeextracellulardopamine(DA).Thereuptakeofthesetwoneurotransmittersisessentialinregulatingconcentrationsinthesynapticcleft.NETs,alongwiththeothermonoaminetransporters,playakeyroleindepressionandneurodegenerativediseases(NDD)suchasAlzheimersandParkinsonsdisease.NETisthetargetofmanyantidepressantsandrecreationaldrugs.Inaddition,anoverabundanceofNETisassociatedwithADHD.

Description:

RecombinantCHOK1celllineexpressinghumannorepinephrinetransporter(NET),GenbankaccessionnumberNM_001043.

HostCellline:

CHO-K1

MycoplasmaTesting:

ThecelllinehasbeenscreenedusingthePCR-basedVenor®GeMMycoplasmaDetectionkit(Sigma-Aldrich,#MP0025)toconfirmtheabsenceofMycoplasmaspecies.

GrowthMedia:

ThawMedium3(BPSCat.#60186):Ham’sF-12medium(Hyclone#SH30526.01)supplementedwith10%FBS(Lifetechnologies#26140-079),1%Penicillin/Streptomycin(HycloneSV30010.01)

CompleteGrowthMedium:ThawMedium3(BPSCat.#60186)plus500μg/mlofHygromycinB(LifeTechnologies#10687-010)toensuretherecombinantexpressionismaintained.Cellsshouldbegrownat37°Cwith5%CO2usingcompletegrowthmedium.

Storage/StABIlity:

Immediatelyuponreceipt,storeinliquidnitrogen.

Application(s):

MonitornorepinephrineuptakeactivityandscreenforactivatorsorinhibitorsofNETactivitiesinacellularcontext

Reference(s):

1.Zhou,J.,DrugsFuture.2004;29(12):1235–1244.2.Jorgensen,S.,etal.,JournalofNeurosciencemethods.2008;169:168-176.

Notes:

Contactusforpricing.

FLIPRvalidationdataisavailableuponrequest.

LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.

ScientificCategory:

Neuroscience

ProductType:

CellLine

Datashownislot-specific.Contactusforspecificinformationonotherlots.

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Bpsbioscience/NET – CHOK1 Recombinant Cell Line/60557/2 vials