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Bpsbioscience/CHK1, GST-tag/40039/10 µg

Species: Human
Host Species: Sf9 insect cells
MW: 82 kDa
Genbank #: NM_001274
Tag(s): N-terminal GST-tag
a.a: full length
Background:
CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2).
Description:
Recombinant full-length human CHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_001274.
Synonym(s):
CHEK1, Checkpoint kinase 1
Purity:
≥95%
Specific Activity:
214 pmol/min/µg
Formulation:
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Walworth, N. et al. Nature. 1993 May 27,363(6427):368-71.
2. Walworth, NC. et al. Science. 1996 Jan 19,271(5247):353-6.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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