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Bpsbioscience/CD73, AviHisTag, Biotin Labeled/79127/50 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/CD73, AviHisTag, Biotin Labeled/79127/50 µg
Species:Human
HostSpecies:HEK293
MW:61kDa
Genbank#:NM_002526
Tag(s):C-terminalAvi-His-Tag
a.a:27-547
Description:
HumanCD73,alsoknownas5-nucleotidaseandEcto-5-nucleotidase,GenBankAccessionNo.NM_002526,a.a.27-547withC-terminalAvi-His-tag,expressedinaHEK293cellexpressionsystem.ThisproteinisenzymaticallybiotinylatedusingAviTag™technology.MW=61kDa.
Synonym(s):
5-Nucleotidase,Ecto-5-Nucleotidase
SpecificActivity:

>20nmol/min/µg.

AssayConditions:ExperimentranaccordingtoCD73InhibitorScreeningAssayKit,#72055colorimetricprotocol(15minincubationat37°C).Phosphatestandard(fromEnzo#BML-AK111)wasusedat100µMseriallydilutedhalf-folddowntheplate.CD73[B]usedat0.3ng/µlandseriallydilutedhalf-fold.

Formulation:
8mMPhosphate,pH7.4,110mMNaCl,2.2mMKCl,and20%glycerol.
Storage/Stability:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.Storingdilutedproteinisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):

1.Deaglio,S.etal.,J.Exp.Med.2007;204(6):1257-1265.

2.Thompson,L.F.,etal.,J.Exp.Med.2004;200(11):1395-1405.

ScientificCategory:
Immunotherapy
ProductType:
Protein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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