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Bpsbioscience/PLK3, Histag/40035/10 µg
Species:Human
HostSpecies:Sf9insectcells
MW:36.7kDa
Genbank#:NM_004073
Tag(s):N-terminalHis-tag
a.a:58–339
Background:
PLK3orPolo-likekinase3isacytokine-induciblekinasethatisamemberofthePolofamilyofserine/threonineproteinkinases.PLK3containsbothacatalyticdomainandaputativeregulatorydomainandplaysanimportantroleintheregulationofcellcycleprogressionandtumorigenesis.PLK3iscloselyassociatedwithcentrosomesinseveralhumancelllinesanditslocalizationisdependentontheintegrityofmicrotubules.Hence,PLK3isalsoinvolvedinregulatingmicrotubuledynamicsandcentrosomalfunction.
Description:
HumanPlk3(GenBankAccessionNo.NM_004073),(a.a.58-339)withN-terminalHis-tag,MW=36.7kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.Source:BaculovirusinfectedSf9cellsPurity:≥80%bySDS-PAGE.SpecificActivity:500U/mg.Oneunitisdefinedastheamountofenzymethatwillphosphorylate1nmolofcaseinsubstrateperminuteatpH7.4and30°C.Assaybuffer:50mMHEPES,pH7.4,3mMMgCl2,3mMMnCl2,1mMDTT,3µMNaorthovanadate,0.5mMATP.
Synonym(s):
CNK,FNK,PRK,polo-likekinase3
SpecificActivity:
350pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillphosphorylate1nmolofsubstrateperminuteatpH7.5and30°C.
AssayConditions:
Enzymereactionisconductedinabuffercontaining50mMHEPES(pH7.5),10mMMgCl2,1mMEGTA,200µMATP,0.01%Brij-35and2µMsubstrate(SER/THR16,Invitrogen)atroomtemperaturefor1hour.
Formulation:
50mMTris-HCl,pH8.0,138mMNaCl,2.7mMKCl,0.05%Tween-20,50%glyceroland1mMDTT.
Format:
Aqueousbuffersolution
Storage/Stability:

>6monthsat-80°C.

Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Li,Z.,etal.,J.Biol.Chem.280(17),16843-16850(2005).
2.Xie,S.,etal.,Oncogene23(21),3822-3829(2004).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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