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Bpsbioscience/Human Thymic stromal lymphopoietin/90250B/10 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/Human Thymic stromal lymphopoietin/90250B/10 µg

Species:Human
HostSpecies:E.coli
MW:15kDa
Genbank#:Q969D9
a.a:29–159
Background:
Thymicstromallymphopoietin(TSLP)promotesthegrowthandactivationofBcells.Italsohaswide-rangingimpactsonbothhematopoieticandnonhematopoieticcelllineages,includingdendriticcells,basophils,eosinophils,mastcells,CD4?,CD8?andnaturalkillerTcells,andepithelialcells.TSLPplaysaroleinthepromotionofTH2responsesinlung-andskin-specificallergicdisorders,anditalsoisinvolvedintheblockadeofTH1/TH17responsesandthepromotionofcancerandautoimmunity.
Description:
RecombinantTSLPisadisulfide-linkedmonomericproteinconsistingof132aminoacidresidues,andmigratesasanapproximately15kDaproteinundernon-reducingconditionsandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanTSLPmaturechainwasexpressedinE.coli.
UniProt
Q969D9
Synonym(s):
TSLP,Thymicstromallymphopoietin
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50wasdeterminedbythedose-dependentproliferationofhumanIL-7RαandhumanTSLPRco-transfectedmouseBaF3cellstobe<3.0ng/ml.
Formulation:
Lyophilizedfroma0.2µmfiltered1mg/mlsolutionof20mMphosphatebuffer,pH7.4,150mMNaCl.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:

Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.

Reference(s):
1.ZieglerSF,etal.AdvPharmacol.2013,66:129-55.
2.YuX,etal.CellImmunol.2012Oct,279(2):174-9.
3.RoanF,etal.JLeukocBiol.2012Jun,91(6):877-86.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
YDFTNCDFEKIKAAYLSTISKDLITYMSGTKSTEFNNTVSCSNRPHCLTEIQSLTFNPTAGCASLAKEMFAMKTKAALAIWCPGYSETQINATQAMKKRRKRKVTTNKCLEQVSQLQGLWRRFNRPLLKQQ
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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