BRD3isincubatedwithbiotinylatedacetyl-histonepeptidesubstrate(BPS#33000)inBRDassaybuffer(BPS#33001)ina10µlreactionfor1houratRT.AlphaLISA®®NickelChelateacceptorbeads(Perkinelmer)areadded,followedbyAlphascreen®®Streptavidin-conjugateddonorbeads(PerkinElmer).After1hourminuteincubationatRT,Alphacountsaremeasured.