Bpsbioscience/Turbonuclease/50310/50,000 units

Ultra-pureversionofBenzonase.Nonspecificendonuclease,hydrolyzesbothsingle-anddouble-strandednucleicacids(DNAandRNA)to5’-phosphorylatedoligonucleotidesof1-4basesinlength.MW=27kDa(homodimer).ExpressedinE.coli.Nodetectableproteaseactivity.TurboNucleasecanbeusedto
•reduceviscosityofcelllysates
•reducebackpressureofcolumnloading
•removenucleicacidcontaminationfromsamplepreparations
•reducenucleicacidcontaminationofNicolumnpurification
•reducesmearinginSDS-PAGEwhenusedwith10%SDSorGelLoadingDye
•makewholecelllysates
•reduceorpreventclumpingofconcentratedcellsandthawedcells
•replacecrudeDNaseIinmanyapplications

Benzonase

≥99%

250units/μlin50mMTris-HCl,pH8.0,50mMNaCl,5mMMgCl₂and50%glycerol

Totalendotoxinlevelis<0.25EU/1,000unitsofTurboNucleaseasdeterminedbytheLALGel-ClotAssay.Noproteaseactivitywasdetected.

≥1.3x10⁶units/mg(>3x10⁶Kunitzunits/mg)

Oneunitconverts1OD260ofsalmonspermDNAintoacidsolublenucleotidesin30minutesat37°C

Reactionbufferof50mMTris-HCl,pH8.0and1mMMgCl₂.

Toreduceviscosityofcelllysate,10-500unitsofTurboNucleasecanbeusedforeachgramofcellpaste.Generally,addingTurboNucleasetocelllysateat25U/mlissufficienttoreducelysateviscosity.Theefficiencyofviscosityreductionmayvarywithbuffers,celltypes,andcelllysismethodsused.Duetoitshighspecificactivity,thetotalamountTurboNucleaseaddedislessthan0.1μg/mloflysateandwillnotcomplicateanydownstreamprocesses.

>6monthsat–20°C.Retains>80%activityafterstorageatRTforover65hours.

Idealtodigestnucleicacidsandtoreduceviscosityduringproteinpurificationandsamplepreparation.

Avoidfreeze/thawcycles

Miscellaneous

Enzyme