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Bpsbioscience/BRD4 (BD1+BD2), Histag/31045/100 µg
Species:Human
HostSpecies:E.coli
MW:47.5kDa
Genbank#:NM_058243
Tag(s):N-terminalHis-tag
a.a:49-460
Description:
HumanBromodomaincontaining4,alsoknownasBRD4,HUNK1,orMCAP,GenBankAccessionNo.NM_058243,a.a.49-460correspondingtobromodomains1and2,withN-terminalHis-tag,MW=47.5kDa,expressedinanE.coliexpressionsystem.
UniProt
O60885
Synonym(s):
Bromodomaincontaining4,BRD4,HUNK1,MCAP
AssayConditions:

BRD4proteinisincubatedwithbiotinylatedacetyl-histonepeptidesubstrate(Cat.#33000)inBRDassaybuffer(Cat.#33001)ina10µlreactionfor1houratRT.AlphaLISA®®NickelChelateacceptorbeads(PerkinElmer)areadded,followedbyAlphascreen®®Streptavidin-conjugateddonorbeads(PerkinElmer).After1hourincubationatRT,Alphacountsaremeasured.

Formulation:
45mMTris-HCl,pH8,124mMNaCl,2.4mMKCl,and10%glycerol.
Format:
Aqueousbuffersolution
Storage/Stability:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofbromodomainbindingassays,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Rodriguez,RM,etal.,JMolMed(Berl).2012May;90(5):587-95.
2.Voigt,P,etal.,GenomeBiol.2011Nov29;12(11):133.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Bromodomain
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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