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Bpsbioscience/BACE1 FRET Assay Kit/71656/96 reactions
Description:

Bace1(β-secretase1)isanasparticproteasethatisinvolvedintheprocessingoftheAmyloidprecursorprotein(APP).CleavageofAPPbyBACE1followedbyγ-secretaseresultsinβ-amyloidpeptideproduction,whichultimatelyleadstoxicAβaccumulation.InAlzheimer’sdisease(AD),ithasbeenwidelyacceptedthatAβaggregationplaysacriticalroleinADpathogenesis,suggestingthatBACE1couldbeapotentialtargettotreatAD.TheBACE1FRETAssayKitisdesignedtomeasureBACE1activityforscreeningandprofilingapplicationsbasedonfluorescenceresonanceenergytransfer(FRET)usingalabeledpeptidesubstrate(below).

BACE1 FRET Assay Kit

Synonym(s):
beta-siteamyloidprecursorproteincleavingenzyme1,ASP2
SuppliedAs:
TheBACE1FRETAssayKitcomesinaconvenient96-wellformat,withenoughpurifiedrecombinanthumanBACE1enzyme,BACE1FRETpeptidesubstrate,andassaybufferfor100enzymereactions.
Format:

COMPONENTS:

Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/Stability:
Atleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Reference(s):
Ghosh,A.K.,etal.J.Neurochemistry120(suppl.1):71-83(2012)
Notes:

MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
MicroplatereadercapableofreadingfluorescenceAdjustablemicropipettorandsteriletips

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Neuroscience
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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