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Bpsbioscience/RIPK2, GSTtag/40173/10 µg
Species:Human
HostSpecies:Sf9insectcells
MW:59kDa
Genbank#:NM_003821
Tag(s):N-terminalGST-tag
a.a:1–299
Background:
RIPK2(RIP2;RICK)isadeathdomain-containingproteinkinaseencodingapredicted540-aminoacidproteinwhichcontainsanN-terminalserine/threoninekinasecatalyticdomainandaC-terminalcaspaseactivationandrecruitmentdomain.RIPK2isthoughttoregulateapoptosisinducedbytheFASreceptorpathway(1).RIPK2hasbeenshowntospecificallyinteractwiththeCARDofICE/caspase-1andthisinteractioncorrelateswiththeprocessingofpro-caspase-1andtheformationoftheactivecaspase-1p20(2).
Description:
RecombinanthumanRIPK2(GenBankAccessionNo.NM_003821),(a.a.1-299)withN-terminalGst-tag,MW=59kDa,expressedinanbaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
RICK;RIP2;CARD3;CARDIAK;CCK;GIG30
Purity:
≥75%
SpecificActivity:
20pmol/min/μg
AssayConditions:

AssayBuffer:25mMMOPS,pH7.2,12.5mMß-glycerophophate,25mMMgCl2,5mMEGTA,2mMEDTA.IncubateRIPK2withsubstrate(0.2mg/ml)and[32P]-ATPat30°Cfor15minutes,thenspotreactiononP81paper,fixin1%phosphoricacid,andassaywithascintillationcounter.

Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/Stability:

>6monthsat-80°C.

Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Inohara,N.etal:RICK,anovelproteinkinasecontainingacaspaserecruitmentdomain,interactswithCLARPandregulatesCD95-mediatedapoptosis.J.Biol.Chem.273:12296-12300,1998.Note:Erratum:J.Biol.Chem273:18675only,1998.
2.Thome,M.etal:IdentificationofCARDIAK,aRIP-likekinasethatassociateswithcaspase-1.Curr.Biol.8:885-888,1998.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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